Method for identifying and screening heat-resistant bottle gourd stocks
A technology for gourd and rootstock, applied in the field of heat resistance identification of gourd type rootstock materials, can solve problems such as low accuracy, unsatisfactory, environmental impact, etc., and achieve the effects of low cost, short time consumption, and accurate results
Inactive Publication Date: 2014-05-14
NINGBO ACAD OF AGRI SCI
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Problems solved by technology
At present, the heat resistance identification of gourd rootstocks mostly uses the field natural identification method, which is easily affected by the environment, has low accuracy, takes a long time, and cannot meet the requirements for rapid screening of target materials in the process of variety selection.
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[0007] The specific implementation of the present invention will be described by taking 37 identifications of heat resistance of gourd and screening heat-resistant materials as an example.
[0008] (1) High temperature seed germination of gourd gourd
[0009] Select 37 portions of gourd gourd material, full and disease-free normal seeds, 90 each, first blanch in 55-60℃ water for 20 minutes, and then soak the seeds in 30℃ warm water for 12 hours. After soaking the seeds, take out the seeds and drain the water, evenly place them in a petri dish (PL-9) spread with four layers of gauze, with a germination density of 15 grains per petri dish, and moisturize with deionized water. Divide into two groups (45 grains / group, 3 petri dishes / group), and germinate in the dark at 30-35°C and 40-45°C. According to the 1996 edition of the International Seed Inspection Regulations, the germination standard is when the seed germ is half as long as the seed. Rinse the seeds with deionized water once...
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The invention relates to a complete method for identifying and screening heat-resistant bottle gourd stocks, which belongs to the field of biology. Rapid screening of heat-resistant materials for variety breeding is based on establishment of a reliable heat resistance identification method. The natural field identification method is susceptible to environment and unable to accurately screen target materials. Therefore, inventing a complete method for rapidly and accurately identifying bottle gourd stock heat resistance by artificial stressing and statistical means is urgent. According to the technical scheme, the complete method includes: using bottle gourd high-temperature seeds to sprout and testing electric conductivity in the seedling stage; calculating identification indexes, namely relative germination rate (40-45 DEG C / 30-35 DEG C), relative germination index (40-45 DEG C / 30-35 DEG C) and relative electric conductivity (6h / 0h); and identifying and screening the bottle gourd seeds by the clustering function of software SPSS13.0. The complete method is simpler in operation flow, consumes less time, is lower in cost, and can be used for identifying heat resistance of the bottle gourd stock material more quickly, more accurately and more conveniently.
Description
1. Technical Field [0001] The invention belongs to the biological field, and particularly relates to a heat resistance identification method of gourd type rootstock materials. 2. Background technology [0002] Bottle gourd (Bottle gourd, Lagenaria siceraria (Molina) Standl.), also known as bottle gourd, flat cattail, and gourd, belongs to the cucurbitaceous cucurbitaceous annual climbing herb. It has a well-developed root system and is used as a grafting rootstock for important melon crops such as cucumber, watermelon and melon, which can effectively improve the adaptability of the plant to adverse environments. In my country, the summer open field temperature in most areas is above 35℃, while the protected area temperature is as high as 40℃. High temperature stress is the main factor restricting the summer cultivation and annual supply of melon crops. Breeding varieties of gourd rootstocks with strong heat resistance and increasing the heat resistance of melon crops through gra...
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IPC IPC(8): A01C1/00A01G7/00
Inventor 王毓洪宋慧应泉盛王迎儿张香琴严蕾艳张华峰
Owner NINGBO ACAD OF AGRI SCI