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Drug delivery system for treating nasopharyngeal carcinoma (NPC) and construction method and application method thereof

A delivery system and technology for nasopharyngeal carcinoma, applied in the field of biomedicine, can solve problems such as unsatisfactory transfection effect of non-viral vector, and achieve the effect of avoiding metabolic problems and high transfection efficiency

Inactive Publication Date: 2012-08-01
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the vectors used in clinical trials of gene therapy are viral vectors. However, due to the safety, ethics, and immunogenicity of viral vectors, the transfer of non-viral vectors (such as liposomes, polymers, etc.) Dyeing effect is not ideal

Method used

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  • Drug delivery system for treating nasopharyngeal carcinoma (NPC) and construction method and application method thereof
  • Drug delivery system for treating nasopharyngeal carcinoma (NPC) and construction method and application method thereof
  • Drug delivery system for treating nasopharyngeal carcinoma (NPC) and construction method and application method thereof

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Experimental program
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Embodiment 1

[0029] It mainly includes: construction of HA / DZI / CPPs drug delivery system (preparation of HA suspension, binding experiment of nano-hydroxyapatite particles and DNAzyme, combination of DNAzyme / HA complex and cell penetrating peptide) and gene Components of the transfection experiment.

[0030] Construction of HA / DZI / CPPs drug delivery system

[0031] 1. Preparation of HA nanoparticle suspension

[0032] The prepared HA nanoparticles were divided into three groups, one group was blank HA particles, one group was modified by PEI, and one group was modified by arginine. HA nanoparticles were made into a 25mg / ml solution with deionized water. Disperse with ultrasonic wave (Ultrasonic Hemogenizer-4710, USA) for 60min, and let stand for 2h. The HA nanoparticle suspension was milky without stratification. Put the suspension into a 50ml glass bottle and store it after autoclaving.

[0033] 2. Binding experiments of nano-hydroxyapatite particles with deoxyribozymes and cell-pene...

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Abstract

The invention discloses a drug delivery system for treating nasopharyngeal carcinoma (NPC) and a construction method and an application method thereof. Hydroxyapatite (HA) with high biocompatibility is used as a carrier, deoxyribozyme capable of targeting NPC latent membrane protein 1 (LMP1) is packaged on HA particles, a deoxyribozyme / HA complex is combined with cell-penetrating peptides (CPPs), and the drug delivery system capable of targeting NPC LMP1 is constructed. The drug delivery system can mediate the deoxyribozyme and plays a key role in the gene therapy of NPC.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the construction and application of a drug delivery system for gene therapy of nasopharyngeal carcinoma. Background technique [0002] Nasopharyngeal carcinoma (nasopharyngeal carcinoma, NPC) is a malignant epithelial tumor with differences in ethnic and geographical distribution. The incidence rate in southern Chinese population is more than 100 times higher than that in Caucasian population, at 25-30 / 100,000. The tumor is a polygenic disease associated with genetic predisposition, EBV infection. Epidemiological studies have shown that EBV infection is very widespread, and the infection rate of adults can reach 98%; in most cases, EBV exists in a latent infection state after infecting the host, and EBNA1, LMP1, LMP2 and other viruses are mainly expressed in nasopharyngeal carcinoma Protein and non-coding RNA (EBV-encoded RNA, EBER). Studies have confirmed that LMP1 (Latent membrane pro...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P35/00
Inventor 黄苏萍周科朝孙仑泉陈燕杨力芳
Owner CENT SOUTH UNIV
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