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Method for improving saffron pigment synthesizing capability of saffron cells

A technology of saffron pigment and saffron, which is applied in the direction of plant cells, can solve the problem of low product yield, and achieve the effects of increasing yield, promoting ability, and stabilizing saffron pigment

Inactive Publication Date: 2012-08-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic researchers have successively carried out research work on the production of crocin by saffron cell culture, but the main problem is that the product yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Choose healthy saffron bulbs, peel off the corm skin, wash the bulbs and rinse under running water overnight. Soak in 75% (v / v) ethanol for 1 min, then disinfect with 0.1% mercuric chloride solution for 15 min, and then rinse with sterile water for 4 times. Blot the water on the surface of the corm with sterilized filter paper and cut into 0.5-1cm 3 of small pieces. Inoculate on MS medium containing 2.0mg / L 2,4-D and 0.5mg / L 6-BA, and culture at 20±1°C for about 35 days to obtain saffron bulb callus.

[0023] The obtained saffron bulb callus was inoculated on the MS medium, wherein additional 0.5mg / L 6-BA, 2mg / LNAA, 30g / L sucrose and 6g / L agar were cultivated in the dark at 20±1°C, every Subculture once every 25-30 days.

[0024] The saffron bulb callus subcultured for 26 to 28 days was inoculated into 1 / 2B5 medium containing 60mg / L chitooligosaccharide, which was supplemented with 0.5mg / L 6-BA, 2mg / L IAA and 20g / L sucrose , Shaking and culturing at 20±1°C in the da...

Embodiment 2

[0027] The saffron corm callus of subculture 26~28 days among the embodiment example 1 is inoculated in 1 / 2B5 medium, wherein additional 0.5mg / L 6-BA, 2mg / L IAA and 20g / L sucrose, at 20± Shake culture at 1°C in the dark at a rotational speed of 130r / min. On the 4th day of culture, chitosan oligosaccharide was added with a final concentration of 60mg / L, and the culture was continued until 26 days, and the content of saffron pigment was increased by 121.3% compared with the control.

Embodiment 3

[0029] The saffron corm callus of subculture 26~28 days among the embodiment example 1 is inoculated in 1 / 2B5 medium, wherein additional 0.5mg / L 6-BA, 2mg / L IAA and 20g / L sucrose, at 20± Shake culture at 1°C in the dark at a rotational speed of 130r / min. On the 14th day of culture, chitosan oligosaccharide was added with a final concentration of 60 mg / L, and the culture was continued until 26 days, and the content of saffron pigment was increased by 85.7% compared with the control.

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Abstract

The invention discloses a method for improving saffron pigment synthesizing capability of saffron cells, which includes the following steps: (1) inoculating saffron calluses onto a plant cell culture medium containing 0.5 mg / L of cytokinin and 2 mg / L of archusia at temperature of 20+ / -1 DEG C for 25-30 days; and (2) culturing the calluses cultured in the step (1) in a plant cell culture medium containing 60 mg / L or 20 mg / L of oligosaccharide, and adding 0.5 mg / L of cytokinin and 2 mg / L of archusia at temperature of 20+ / -1 DEG C for 26 days. The method can remarkably improve synthesizing capability of saffron cells and content of saffron pigment, and the obtained saffron pigment is stable and not apt to brown.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for improving the synthesis of saffron pigment by saffron cells. Background technique [0002] Saffron (Crocus sativus L.), also known as saffron, saffron, is a bulbous perennial herb of the genus Crocus in the family Iridaceae. It is native to southern Europe and Iran, and was introduced to Tibet through India, so it is called saffron. Saffron has been used as a precious seasoning, spice and dye in Europe for hundreds of years. Saffron also has high medicinal value and is a precious medicinal material. As a medicinal product abroad, it was first recorded in the "Ebers Papyrus" around 1550 AD. As a traditional Chinese medicine, it has been used for a long time in my country. In "Compendium of Materia Medica", saffron is its name. Chinese traditional Chinese medicine believes that saffron has the functions of promoting blood circulation and removing blood stasis, relieving...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 袁丽红孙镇吴频梅
Owner NANJING UNIV OF TECH
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