Human rotavirus strain and separation, culture and identification method thereof

A rotavirus, strain technology for viruses/phages, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as high cost of cold chain storage system requirements, intussusception and virulence reversion mutations , to achieve the effect of improving primary culture efficiency, high virus recovery yield, and high impurity removal effect

Inactive Publication Date: 2012-08-01
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the rotavirus vaccines in preclinical research or on the market are all oral attenuated live vaccines. Although the live attenuated vaccines have certain effects, they have defects in safety, such as causing intussusception and virulence regression mutations and producing new viruses. The risk of mutant stra

Method used

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  • Human rotavirus strain and separation, culture and identification method thereof
  • Human rotavirus strain and separation, culture and identification method thereof
  • Human rotavirus strain and separation, culture and identification method thereof

Examples

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Embodiment 1

[0039] Example 1. Rotavirus ( Rotavirus A ) Isolation, cultivation and identification of ZTR-5 strain

[0040] Sample source

[0041] The original samples were collected from inpatient and outpatient cases in the Department of Pediatrics, Zhaotong Hospital, Yunnan Province in the fall of 2009, and diarrhea excrement specimens from children with diarrhea. The ZTR-5 strain was derived from a sample of a 4-month-old male with diarrhea. The symptoms of this case were moderate diarrhea, vomiting, and yellowish watery stool. He was hospitalized on October 5, 2009. The course of the disease was 4 days. The sample was collected in 2009. On October 6, 2009, the child's condition improved after receiving infusion treatment and was discharged from hospital on October 9, 2009. He had no previous medical history. The rotavirus antigen was detected in the excreta. The confirmed case was an acute rotavirus infection. diarrhea.

[0042] 1. Isolation method of ZTR-5 strain:

[0043] (1) Obtain vir...

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Abstract

The invention discloses a human rotavirus strain. The human rotavirus strain is characterized in that the strain is a Rotavirus A ZTR-5 strain, and is collected to CGMCC (China General Microbiological Culture Collection Center of Microbiological Culture Management Committee) on 27th, June 2011, and the number is CGMCCNo.4977. The strain is a wild type single purified strain of human rotaviruses, and is strong in virus replication capability and good in genetic stability; and the virus seed can be applied as a production virus seed for developing human rotavirus vaccines, and is applicable to attenuated type rotavirus oral vaccines and inactivated human rotavirus inject vaccines. The invention provides a separation, culture and identification method of the human rotavirus strain.

Description

technical field [0001] The invention relates to the separation and identification of rotavirus, especially human rotavirus seed strain and the immunological effect evaluation of the strain. Background technique [0002] Human rotavirus is one of the most important pathogens causing diarrhea in infants and young children worldwide. Among infants under the age of 5 in the world, there are 17 million cases of acute gastroenteritis caused by rotavirus infection every year, causing about 40,000 infant deaths, and 80% of these deaths occur in low-income countries in Asia and Africa. income in developing countries. In my country, the positive rate of rotavirus detection among infants under 5 years old hospitalized due to diarrhea is about 50%. Rotavirus infection in infants and young children mainly manifests as sudden onset, watery stools, vomiting, fever and dehydration, and eventually severe dehydration and electrolyte imbalance lead to death in severely ill infants and young ...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N7/08G01N33/569C12Q1/70C12Q1/68C12R1/93
Inventor 孙茂盛李泓钧吴晋元张光明易山
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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