Early evaluating method for tea tree winter resistance
An identification method, cold resistance technology, applied in the field of botany, can solve the problems of inaccuracy and reliability
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[0042] 1. Treatment of test materials
[0043] From October, as the climate gradually turns from autumn to winter, the temperature gradually drops. Two varieties of Shuchazao and Wuniuzao are taken from the tea garden of Dayang Town, Hefei City, Anhui Province every month, and the content of the leaves in the leaves is determined by high performance liquid chromatography. Sucrose content, continued until January of the following year, and compared the changes in the sucrose content of the two varieties.
[0044] 2. Determination of sucrose content
[0045] A. Sample Extract Preparation
[0046] 1) Take 25g of mature fresh leaves of tea tree and cut them into 0.2-0.5cm 2 debris;
[0047] 2) Add the shredded leaves in step 1) into 250 mL of pure water, and extract them in a water bath at 90° C. for 1 hour under sealed conditions; the percentage by weight of leaves and pure water extraction is 1:10;
[0048] 3) Take out the extraction solution in step 2) and cool it to room tem...
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