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Functional mycobacterium tuberculosis antigen polypeptide and application thereof

A technology for tuberculosis, active ingredients, applied in the field of functional Mycobacterium tuberculosis antigen polypeptides

Active Publication Date: 2012-09-05
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of tuberculosis research, there is no report on the direct screening of Mycobacterium tuberculosis antigens from lung tissue

Method used

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  • Functional mycobacterium tuberculosis antigen polypeptide and application thereof
  • Functional mycobacterium tuberculosis antigen polypeptide and application thereof
  • Functional mycobacterium tuberculosis antigen polypeptide and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Example 1. Functional Mycobacterium tuberculosis antigen polypeptide stimulates mouse spleen and lymph node cell proliferation

[0015] The functional Mycobacterium tuberculosis antigen polypeptide used in this embodiment is Pep1 (fadB5-1) and Pep2 (fadB5-2), the amino acid sequence of Pep1 is shown in sequence 1 in the sequence listing; the amino acid sequence of Pep2 is shown in the sequence in the sequence listing 2.

[0016] 1. Preparation of Pep1 and Pep2

[0017] Pep1 and Pep2 were synthesized from the carboxy-terminus to the amino-terminus by the solid-phase peptide synthesis method (Fmoc method). Wherein, the resin used is 2-CL-Trt with a degree of substitution of 0.5. The synthesized polypeptides were purified by high performance liquid chromatography to obtain Pep1 and Pep2 with a purity of 98%. Purified Pep1 and Pep2 were identified by mass spectrometry. The amino acid sequence of Pep1 is shown in sequence 1 in the sequence listing; the amino acid sequence...

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Abstract

The invention discloses a functional mycobacterium tuberculosis antigen polypeptide and an application thereof. According to the polypeptide disclosed by the invention, an amino acid sequence is represented by a sequence 1 in a sequence table. The polypeptide can stimulate H37Rv infected mice spleen cells and lymphocytes to be obviously multiplied so as to be subjected to an antigen-antibody reaction with infected mice blood serum; and therefore, the polypeptide has the antigen property and can be used for developing novel tuberculosis preventing and treating vaccines.

Description

technical field [0001] The present invention relates to functional Mycobacterium tuberculosis antigen polypeptide and its application. Background technique [0002] According to WHO statistics, nearly 1 / 3 of the world's population is infected with Mycobacterium tuberculosis (M.tb). For several years, tuberculosis ranks the top two in the incidence and death of infectious diseases in my country. Tuberculosis is already an infectious disease that seriously endangers people's health and the national economy, and China is a country with a high burden of tuberculosis, so the formulation of tuberculosis prevention and control strategies has become a major issue related to my country's national economy and people's livelihood. The treatment of tuberculosis is currently the most widely used "chemotherapy". The treatment of chemical drugs has obvious effects on tuberculosis. With the increase of morbidity, chemotherapy has shown its limitations. Then, new prevention and control met...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/31C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N5/078A61K39/04A61K48/00A61P31/06
Inventor 于杨刘海鹰张艳金奇
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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