Helianthus tuberosus fructan-exohydrolases (Ht-FEH) gene and application thereof

A technology of fructan and hydrolase, which is applied in the field of genetic engineering and can solve problems that have not been reported
CN102653769BInactive Publication Date: 2014-09-10NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2014-09-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of gene engineering and discloses a helianthus tuberosus fructan-exohydrolases (Ht-FEH) gene and application of the Ht-FEH gene. The gene Ht 1-FEH has a nucleotide sequence shown in the SEQ ID NO.1 and is used for coding Ht 1-FEH with an amino acid sequence shown in the SEQ ID NO.2. The gene Ht 1-FEH can be applied to production of fructose substances through hydrolysis of inulin or cultivation of transgenic salt-tolerant plants.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering and relates to a Jerusalem artichoke fructan exohydrolase gene and application thereof. Background technique

[0002] Plant fructans are mainly carbohydrates composed of multiple fructosyl groups connected by glycosidic bonds. Although fructans can be found in 15% of angiosperms, the content of fructans in plants is generally low, only in the tubers of Jerusalem artichoke (Helianthus tuberosus); chicory (Cichorium intybus), burdock (Arctium lappal) and chicken Roots of footgrass (Dactylis glomeratus); bulbs of lilies (Lilium brownii) and onions (Allium cepa); tissues of a few plants such as stems and leaves of ryegrass (Lolium perenne), barley (Hordeum vulgare), and wheat (Triticum aestivum) Relatively high levels in medium (Ritsema and Smeekens 2003a; Van Laere and Van Den Ende 2002).

[0003] Sucrose is a precursor for fructan synthesis (Van Laere and Van Den Ende 2002). There are mainly tw...

Claims

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