Somatic embryogenesis method of phyllostachys heterocycla var. pubescens

An embryogenesis and somatic cell technology, applied in the field of forest biotechnology and tissue culture, can solve the problems of poor repeatability, difficult culture, and little progress.

Inactive Publication Date: 2012-09-12
袁金玲 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] Ogita used Ph.nigra var.henosis bamboo shoots to induce callus in 2005, but the callus was prone to browning and no regeneration (reference 10)
[0043] Throughout the current research status of bamboo plant regeneration systems at home and abroad, so far the regeneration system is mainly built on cluster bamboo species, there are few reports on scattered bamboo, and the efficiency is low and the repeatability is poor. The most important scattered bamboo has not been seen. Species-Moso Bamboo Regeneration System Report
Scattered bamboo species are difficult to cultivate due to their different physiological habits from clustered bamboo. Although many units have carried out research, there is little progress

Method used

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Embodiment Construction

[0049] The present invention will be described in detail below in conjunction with the example of Phyllostachys heterocycla var. pubescens.

[0050] (1) Select plump and healthy seeds, first process 1 minute with 75% (v / v) ethanol; then process 15-20 minutes with 0.2% sodium hypochlorite that adds 5-6 drops of Tween-80 per liter; Rinse several times with sterile water; finally blot the surface moisture with sterile paper and wait for inoculation;

[0051] (2) Inoculate the sterilized explants on the callus induction medium, the induction medium is based on MS medium, add 4mg / L of 2,4-D, 0.1mg / L of ZT, 10g / L of carrageenan, 500mg / L of proline, 500mg / L of glutamine, 300mg / L of hydrolyzed casein, cultured in the dark at 26°C for 20-30 days, the explants formed a callus the size of a grain of rice; The pale yellow compact callus was isolated and inoculated on the subculture medium. The proliferation medium was based on MS medium, adding 2mg / L of 2,4-D, 10g / L of carrageenan, 500m...

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Abstract

The invention discloses a somatic embryogenesis method of phyllostachys heterocycla var. pubescens, which includes the following steps that an explant of phyllostachys heterocycla var. pubescens is appropriately disinfected and then is induced on an inductive medium to produce a callus tissue; after being processed with multiplication culturing, the callus tissue firstly forms an embryoid on a differential medium and then the embryoid bourgeons and forms a regenerated plant on a germination medium; finally the regenerated plant is processed with the cultivations of seedling toughening up and seedling exercising and then is transplanted to survive. The invention solves the technical difficulty in somatic embryogenesis of phyllostachys heterocycla var. pubescens and provides a technology platform for the technical breeding of scattered bamboo plants.

Description

technical field [0001] The invention relates to the fields of tree biotechnology and tissue culture, in particular to somatic cell embryogenesis of Phyllostachys pubescens. Background technique [0002] Since the 1980s, with the rapid development of plant biotechnology in the world, the research on bamboo biotechnology has gradually increased at home and abroad. At present, the international reports on the bamboo regeneration system related to this patent mainly include: [0003] In 1982, Metha et al. reported that mature embryos of Bambusa arundinacea induced callus and regenerated plants (Reference 1). [0004] In 1983, Huang and Murashige induced callus from leaves and shoot tips of Phyllostachy, Sasa, and Bambusa (Reference 2). [0005] In 1985, Rao et al. induced callus with mature seeds of male bamboo (Dendrocalamus.strictus), and then regenerated plants (Reference 3). [0006] In 1986, Yeh and Chang used the inflorescences of green bamboo (B.oldhamii) and hanging si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 袁金玲顾小平岳晋军
Owner 袁金玲
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