Somatic embryogenesis method of phyllostachys heterocycla var. pubescens

Abstract

The invention discloses a somatic embryogenesis method of phyllostachys heterocycla var. pubescens, which includes the following steps that an explant of phyllostachys heterocycla var. pubescens is appropriately disinfected and then is induced on an inductive medium to produce a callus tissue; after being processed with multiplication culturing, the callus tissue firstly forms an embryoid on a differential medium and then the embryoid bourgeons and forms a regenerated plant on a germination medium; finally the regenerated plant is processed with the cultivations of seedling toughening up and seedling exercising and then is transplanted to survive. The invention solves the technical difficulty in somatic embryogenesis of phyllostachys heterocycla var. pubescens and provides a technology platform for the technical breeding of scattered bamboo plants.

Description

technical field [0001] The invention relates to the fields of tree biotechnology and tissue culture, in particular to somatic cell embryogenesis of Phyllostachys pubescens. Background technique [0002] Since the 1980s, with the rapid development of plant biotechnology in the world, the research on bamboo biotechnology has gradually increased at home and abroad. At present, the international reports on the bamboo regeneration system related to this patent mainly include: [0003] In 1982, Metha et al. reported that mature embryos of Bambusa arundinacea induced callus and regenerated plants (Reference 1). [0004] In 1983, Huang and Murashige induced callus from leaves and shoot tips of Phyllostachy, Sasa, and Bambusa (Reference 2). [0005] In 1985, Rao et al. induced callus with mature seeds of male bamboo (Dendrocalamus.strictus), and then regenerated plants (Reference 3). [0006] In 1986, Yeh and Chang used the inflorescences of green bamboo (B.oldhamii) and hanging si...

AI Technical Summary

Problems solved by technology

[0011] Ogita used Ph.nigra var.henosis bamboo shoots to induce callus in 2005, but the callus was prone to browning and no regeneration (reference 10)

[0043] Throughout the current research status of bamboo plant regeneration systems at home and abroad, so far the regeneration system is mainly built on cluster bamboo species, there are few reports on scattered bamboo, and the efficiency is low and the repeatability is poor. The most important scattered bamboo has not been seen. Species-Moso Bamboo Regeneration System Report

Scattered bamboo species are difficult to cultivate due to their different physiological habits from clustered bamboo. Although many units have carried out research, there is little progress