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Method for degrading phthalic acid esters in water by using ultramicro bacteria under oligotrophic conditions

A technology for hydrolyzing phthalates and oligotrophs, which is applied in the application field of microbial remediation of polluted water bodies, can solve problems such as limiting the practical application range of microbial remediation technology, and achieve low demand, wide application range, and good treatment effect

Active Publication Date: 2012-09-12
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unlike the high-nutrient culture conditions in the laboratory, most of the water environment on the earth is an oligotrophic environment (DOC is lower than 1mg / L), which limits the practical application range of these methods in microbial remediation technology.

Method used

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  • Method for degrading phthalic acid esters in water by using ultramicro bacteria under oligotrophic conditions
  • Method for degrading phthalic acid esters in water by using ultramicro bacteria under oligotrophic conditions
  • Method for degrading phthalic acid esters in water by using ultramicro bacteria under oligotrophic conditions

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Use Evian water as the base water (pasteurized, pass through a 0.22μm filter membrane), add 1 mg / L of phthalate pollutants (dimethyl phthalate), and then inoculate with Rhodoferax ferrireducens PAE-UM strains, the inoculum concentration was 10 3 count / ml. Then subpackage into 100mL Erlenmeyer flasks, put into shaker and cultivate (30 ℃, rotating speed is 150r / min), utilize flow cytometry to detect bacterial growth and utilize high performance liquid chromatography to detect the degradation status of pollutants, such as attached figure 1 Shown is the degradation growth curve of dimethyl phthalate when the initial concentration is 1mg / L.

Embodiment 2

[0032] Use Evian water as the base water (pasteurized, pass through a 0.22μm filter membrane), add 0.1mg / L of phthalate pollutants (diethyl phthalate), and then inoculate Rhodoferax ferrireducens PAE- UM strains, inoculum concentration at 10 3 count / ml. Then subpackage into 100mL Erlenmeyer flasks, put into shaker and cultivate (30 ℃, rotating speed is 150r / min), utilize flow cytometry to detect bacterial growth and utilize high performance liquid chromatography to detect the degradation status of pollutants, such as attached figure 2 as shown, figure 2 It is the degradation growth curve of diethyl phthalate when the initial concentration is 0.1mg / L.

Embodiment 3

[0034] Use Evian water as the base water (pasteurized, pass through a 0.22μm filter membrane), add 0.1mg / L of phthalate pollutants (dibutyl phthalate), and then inoculate Rhodoferax ferrireducens PAE- UM strains, inoculum concentration at 10 3count / ml. Then subpackage into 100mL Erlenmeyer flasks, put into shaker and cultivate (30 ℃, rotating speed is 150r / min), utilize flow cytometry to detect bacterial growth and utilize high performance liquid chromatography to detect the degradation status of pollutants, such as attached image 3 as shown, image 3 It is the degradation growth curve of dibutyl phthalate when the initial concentration is 0.1mg / L.

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Abstract

The invention discloses a method for degrading phthalic acid esters (PAEs) plasticizer pollutant in water by using ultramicro-bacteria proteus Rhodoferax ferrireducens PAE-UM (CGMCC No.5917) capable of growing under oligotrophic conditions, belonging to the application field of micro-biological degradation and restoration of pollutants in water, solving the problem of degrading the PAEs pollutants in water under oligotrophic conditions that can not be solved in the existing micro-biological degradation methods. The method comprises the following steps: (1) culturing and preparing strains; (2)inoculating and degrading the PAEs pollutants in water; and (3) carrying out detection control on the degradation status of the PAEs pollutants. The method has the advantages of simple process flow, low cost, and wide application range and conditions, and the cultured strains has strong ability to degrade the PAEs pollutants under oligotrophic conditions.

Description

technical field [0001] The method belongs to the applied technical field of microbial remediation of polluted water bodies. It involves a method for degrading and repairing plasticizers of phthalates (PAEs) in water using a kind of ultramicrobacteria that can grow under oligotrophic conditions. The PAEs include 6 representative phthalates ( That is, dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), (2-ethylhexyl) phthalate (DEHP), Di-n-octyl phthalate (DOP) and butylbenzyl phthalate (BBP)). Background technique [0002] Phthalates (PAEs) are an important endocrine disruptor pollutant in water bodies. PAEs are widely used as plasticizers in plastic additives to improve the mechanical properties of plastic resins. The content of PAEs in plastic products is very high, for example, the content of PAEs in PVC plastics is as high as more than 20%. Currently, PAEs account for more than 80% of the global plasticizer market. According to reports, the an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
Inventor 王莹莹马克.巴特兰姆韩东东李蕾孙瑞
Owner NANKAI UNIV
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