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Biosensor and construction method of gene trapping system

A technology of biosensors and construction methods, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as limiting the scope of application of sorting and screening gene types

Inactive Publication Date: 2012-09-12
BEIJING WELLSENS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing methods for sorting host bacteria mainly rely on morphological recognition (based on color and shape changes) or restrictive media (based on unique carbon sources or antibiotics), which limits the scope of its sorting application and the types of genes that can be screened.

Method used

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  • Biosensor and construction method of gene trapping system
  • Biosensor and construction method of gene trapping system

Examples

Experimental program
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Embodiment

[0079] The biosensor provided in this embodiment takes the biosensor Acinetobacter baylyi ADPWH_lux as an example, and its construction method is as follows:

[0080] 1) Construct BamHI and EcoRI restriction endonuclease sites in the salA-salR gene of Acinetobacter baylyi ADP1, and obtain Acinetobacter Acinetobacter through the primers and the BamHI enzyme recognition sites and EcoRI enzyme recognition sites on the primers The 5' part and the 3' part of the salA-salR gene of baylyi ADP1. BamHI and EcoRI restriction endonuclease sites were constructed in the salA-salR gene of Acinetobacter baylyi ADP1, where the chromosomal DNA of Acinetobacter baylyi ADP1 (Barbe et al., 2004) has been fully sequenced and can be obtained from the NCBI Genebank database Get (NC_005966.1). The following primers were designed to obtain the 5' part and the 3' part of the complete salA-salR gene of Acinetobacter baylyi ADP1 by PCR reaction:

[0081] P1: 5'-CTCAAAGGAAATGAGTCGTGGGTA-3'

[0082] P2:...

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Abstract

The invention discloses a biosensor and a construction method of a gene trapping system, and belongs to the technical field of biotechnologies. The biosensor is a transformant or a recombinant constructed by using Acinetobacter baylyi ADP1 as a chassis organism. The construction method of the gene trapping system comprises the steps that gene fragments are trapped; and the trapped gene fragments are sequenced to obtain the sequence of the trapped gene fragments. By adopting the biosensor and the construction method of the gene trapping system, a technology developed on the basis of related technologies of the prior micro genomics, based on the whole-cell biosensor and used for trapping functional genes from environmental samples is provided.

Description

technical field [0001] The invention relates to the technical field of biological transmission technology, in particular to a method for constructing a biosensor and a gene capture system. Background technique [0002] Synthetic biology is one of the latest branches of life sciences. It uses molecular biology methods to systematically transform cellular software (genetic material represented by DNA) and hardware (organelles represented by ribosomes) to make it possess specific function. These living cells can self-replicate and act as "synthetic factories", converting simple and cheap energy and substances (such as sunlight and carbon dioxide) into high value-added drugs, materials and bioenergy, and changing the concentration or toxicity of chemicals As a signal that can be measured, or used to eliminate refractory pollutants. The development of synthetic biology has long-term strategic and realistic strategic significance for solving major economic and technological issu...

Claims

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Application Information

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IPC IPC(8): C12N15/74C12N1/21C12N15/10C12R1/01
Inventor 王允黄巍
Owner BEIJING WELLSENS BIOTECH
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