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New application of tetrandrine in fungal bio-film resistance

A technology of tetrandrine and biofilm, applied in the field of medicine

Inactive Publication Date: 2012-09-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] We found that tetrandrine used alone in vitro can effectively inhibit the growth and proliferation of fungal biofilms, and has a good inhibitory effect on the formed fungal biofilms. So far, there has been no report of tetrandrine alone antifungal biofilms

Method used

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  • New application of tetrandrine in fungal bio-film resistance
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  • New application of tetrandrine in fungal bio-film resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: The effect of Tetrandrine alone on the biofilm of different Candida albicans strains.

[0015] Materials and methods

[0016] 1. Test drug

[0017] Tetrandrine: purchased from Sigma Company.

[0018] Fluconazole: purchased from Sigma Company.

[0019] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0020] XTT: purchased from Sigma Corporation.

[0021] Menadione (menadione): purchased from Sigma Company.

[0022] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0023] Acetone: purchased from Sigma Company.

[0024] Tetrandrine was made into 6.4 mg / ml and 12.8 mg / ml solutions with dimethyl sulfoxide, amphotericin B was made into 4 mg / ml solution with dimethyl sulfoxide, and fluconazole was made into 102.4 mg with dimethyl sulfoxide / ml solution, the test drug was stored at -20°C. Before the experiment, the drug stock solution was taken out and melted in a 35°C incubat...

Embodiment 2

[0056] Embodiment 2: the effect of tetrandrine (also known as tetrandrine) alone on the biofilm of different Cryptococcus neoformans strains

[0057] Materials and methods

[0058] 1. Test drug

[0059] Tetrandrine: purchased from Sigma Company.

[0060] Fluconazole: purchased from Sigma Company.

[0061] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0062] XTT: purchased from Sigma Corporation.

[0063] Menadione (menadione): purchased from Sigma Company.

[0064] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0065] Acetone: purchased from Sigma Company.

[0066] Tetrandrine was made into 6.4mg / ml and 12.8mg / ml with dimethyl sulfoxide, amphotericin B was made into 4mg / ml with dimethyl sulfoxide, and fluconazole was made into 102.4mg / ml with dimethyl sulfoxide , and the test drug was stored at -20°C. Before the experiment, the drug stock solution was taken out and melted in a 35°...

Embodiment 3

[0097] Embodiment 3: the effect of tetrandrine (also known as tetrandrine) alone on the biofilm of different Aspergillus strains

[0098] Materials and methods

[0099] 1. Test drug

[0100] Tetrandrine: purchased from Sigma Company.

[0101] Fluconazole: purchased from Sigma Company.

[0102] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0103] XTT: purchased from Sigma Corporation.

[0104] Menadione (menadione): purchased from Sigma Company.

[0105] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0106] Acetone: purchased from Sigma Company.

[0107] Tetrandrine was made into 6.4mg / ml and 12.8mg / ml with dimethyl sulfoxide, amphotericin B was made into 4mg / ml with dimethyl sulfoxide, and fluconazole was made into 102.4mg / ml with dimethyl sulfoxide , and the test drug was stored at -20°C. Before the experiment, the drug stock solution was taken out and melted in a 35°C incubator,...

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Abstract

The invention discloses new application of tetrandrine in fungal bio-film resistance, and relates to application of tetrandrine in the field of fungal bio-film resisting treatment medicaments. The tetrandrine compound has the following chemical structural formula; and experimental results show that the tetrandrine compound has high fungal bio-film resisting activity in vitro. Experimental results show that the tetrandrine can effectively inhibit growth and proliferation of the fungal bio-films in vitro and effectively inhibit mature bio-films.

Description

Technical field: [0001] The invention relates to the technical field of medicine, and relates to a new application of tetrandrine (also known as tetrandrine) in antifungal biofilms. Background technique: [0002] Tetrandrine (also known as tetrandrine) is derived from the root of Stephania tetrandra S. Moore, a plant of the Tetradaceae family. Its structure is shown in formula (I), and it is a colorless needle-like crystal with a melting point of 217-218°C , It is mainly used clinically to treat early mild hypertension. It has been reported that tetrandrine (also known as tetrandrine) has a synergistic effect on fluconazole against Candida albicans (Li Fengxia, Zhang Hong, Synergistic effect of tetrandrine on fluconazole anti-Candida albicans, Zhonghua Journal of Dermatology, 2006, 39 (8): 454-456), but there is no report on the antifungal biofilm research of tetrandrine (also known as tetrandrine) alone. [0003] [0004] Biofilm is a microbial aggregation group, which...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P3/00
Inventor 王彦赵兰雪李德东曹永兵阎澜姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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