Pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen and application thereof

Abstract

The invention discloses a pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen and application thereof and belongs to the field of biological vaccines. The pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen adopts a strategy of an antigenized antibody, after main antigen epitopes of a plurality of strains of pig foot-and-mouth disease virus O-type are connected in series reasonably, the plurality of strains of pig foot-and-mouth disease virus O-type are coupled with a pig intravenous gamma globulin (IgG) heavy chain constant region to construct the pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen, and after ration through a Bio-Rad protein ration kit, the pig foot-and-mouth disease virus O-type broad spectrum multi-epitope recombination antigen and recombination foot-and-mouth disease virus 3D protein are matched to prepare the vaccines. Animal immunity testing results show that the vaccines can stimulate an organism to generate high-titer protective antibodies when the vaccines are used independently or matched with the recombination foot-and-mouth disease virus 3D protein to be used, an antibody level is higher than a national standard, and good application prospects are achieved.

Description

Technical field [0001] The invention relates to a porcine foot-and-mouth disease virus recombinant antigen and its application, in particular to a porcine foot-and-mouth disease virus O type broad-spectrum multi-epitope recombinant antigen and its application, and also relates to a vaccine prepared from the antigen, belonging to the field of biological vaccines. Background technique [0002] Foot-and-mouth disease, as a major animal disease that infects pigs, cattle and sheep, not only seriously threatens the healthy development of animal husbandry, but also involves the safety of animal-derived food and its export. Once an epidemic occurs, the losses will be heavy and the impact will be severe. Inactivated vaccines, as the main prevention and control substances, play a very important role in controlling the FMD epidemic. However, the production of such vaccines requires the construction of high-level biosafety production workshops to prevent the escape of pathogens, and it is ne...

AI Technical Summary

Problems solved by technology

However, the large-scale animal experiments all ended in failure (the protection rate of the virus attack did not reach the national standard), mainly because the small molecule antigens developed could not stimulate the body to produce a protective level of neutralizing antibodies, and some even could produce a certain level of protection. Antibodies, but the duration is short, which cannot meet the needs of epidemic prevention and control

In addition, the developed viral particle-like capsid protein can induce the body to produce high-titer protective antibodies, and immunized animals can resist the challenge of homologous viruses, but the low yield and high cost of the antigen limit the application of the vaccine

Recently, people have increased the immunogenicity of vaccines by increasing T cell epitopes and foreign carrier proteins to enhance antigen presentation capabilities, increase molecular weight, and promote B cell maturation and differentiation, enhance immune response, and increase the level of peripheral blood circulating antibodies and duration, although the immunogenicity of the antigen has been improved to a certain extent, the anti-foreign carrier protein antibody produced after multiple immunizations has affec

Images