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Artificial cultivation method of Paecilomyces tenuipes (Peck) Samson and pharmaceutical use of Paecilomyces tenuipes (Peck) Ssamson

A technology of artificial cultivation and Paecilomyces pedis, applied in microorganism-based methods, biochemical equipment and methods, drug combinations, etc., can solve the problems of low yield, unstable quality, long production cycle, etc., and achieve the effect of high yield

Active Publication Date: 2014-02-19
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the source of artificial culture of Paecilomyces tenipedes is simple and easy to obtain, which can solve the problems of scarcity of natural medicinal insect fungus resources, long production cycle, low yield, and unstable quality.

Method used

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  • Artificial cultivation method of Paecilomyces tenuipes (Peck) Samson and pharmaceutical use of Paecilomyces tenuipes (Peck) Ssamson
  • Artificial cultivation method of Paecilomyces tenuipes (Peck) Samson and pharmaceutical use of Paecilomyces tenuipes (Peck) Ssamson
  • Artificial cultivation method of Paecilomyces tenuipes (Peck) Samson and pharmaceutical use of Paecilomyces tenuipes (Peck) Ssamson

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Artificial cultivation of Paecilomyces tenabilis.

[0043]Inoculate the Paecilomyces finelegs strain (CGMCC NO.4176) on the PSA slant medium and culture it at 22°C for 10 days; scrape the mycelium from the slant and inoculate it into the liquid medium of the Erlenmeyer flask based on PDB liquid , at 24°C, 140rpm, cultivated for 3 days to prepare the Paecilomyces cicadae shake flask seed liquid; the Paecilomyces tenipedes shake flask seed liquid was inserted into the sterilized PSB liquid by volume percentage as 10% for seed culture Paecilomyces therapeus fermenter seed liquid can be obtained after culturing for 2 days in a fermenter based on the base at 24°C with a stirring rate of 140rpm; the obtained fermenter seed liquid is 5% by weight and inserted into the sterilized In the described solid-phase culture medium, at a temperature of 24°C and a humidity of 70%, during solid fermentation, an artificial scattering light source was added (continuously increasin...

Embodiment 2

[0046] Artificial cultivation of Paecilomyces tenipedes

[0047] Inoculate the Paecilomyces tenipedes strain (CGMCC NO.4176) on the PSA slant medium and culture it at 23°C for 9 days; scrape the mycelium from the slant and inoculate it into the liquid medium of the Erlenmeyer flask based on the PSA liquid , at 25°C, 150rpm, cultivated for 3 days to obtain the Paecilomyces cicadae shake flask seed liquid; the Paecilomyces tenipedes shake flask seed liquid was inserted into the sterilized PSB liquid at a volume percentage of 5% for seed culture Paecilomyces thelipedes fermenter seed liquid can be obtained after cultivating for 2 days in a fermenter based on the base at 25°C with a stirring rate of 150 rpm; In the above-mentioned solid-phase culture medium, at a temperature of 25°C and a humidity of 75%, during solid fermentation, artificial scattering light sources (continuously increasing light intensity 140Lux) were added, and regular ventilation and exhaust (6 hours apart) V...

Embodiment 3

[0050] Artificial cultivation of Paecilomyces tenipedes

[0051] Inoculate the Paecilomyces finelegs strain (CGMCC NO.4176) on the PSA slant medium and culture it at 24°C for 8 days; scrape the mycelia from the slant and inoculate it into the PDA liquid-based Erlenmeyer flask liquid medium , at 26°C, 160rpm, cultivated for 3 days to obtain the Paecilomyces cicadae shake flask seed liquid; the Paecilomyces tenipedes shake flask seed liquid was inserted into the sterilized PSB liquid at a volume percentage of 8% for seed culture Paecilomyces thelipedes fermenter seed liquid can be obtained after culturing for 1 day in a fermenter based on the base at 26°C with a stirring rate of 160rpm; the fermented liquid was added to the sterilized fermented liquid at 10% by weight. In the above-mentioned solid-phase culture medium, at a temperature of 26°C and a humidity of 80%, during solid fermentation, artificial scattering light sources (continuously increasing light intensity 130Lux) we...

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Abstract

The invention relates to an artificial cultivation method of Paecilomyces tenuipes (Peck) Samson and pharmaceutical use of the Paecilomyces tenuipes (Peck) Samson. The artificial cultivation method of the Paecilomyces tenuipes (Peck) Samson disclosed by the invention comprises the following steps: 1) preparing slant strain; 2) preparing shake flask seed liquid; 3) preparing fermentation tank seed liquid; 4) inoculating the fermentation tank seed liquid in solid phase medium, carrying out closed cultivation for 30-35 days in a standing mode, and 5) recovering. The invention also further discloses a cultured product obtained by adopting the cultivation method and pharmaceutical use of the cultured product. The cultured product of the Paecilomyces tenuipes (Peck) Samson provided by the invention has obvious treatment and prevention actions on occurrence and development of hepatic diseases such as chemical liver injury, viral hepatitis, hepatic fibrosis and the like.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines and natural medicines, and in particular relates to a method for artificially culturing Paecilomyces tenabilis and its pharmaceutical use. Background technique [0002] Paecilomyces tenuipes (Peck) Samson] is an anamorph of Cordyceps takaomontana Yakushiji & Kumazawa. Studies have shown that the main chemical components of Paecilomyces tenipedes, such as sterols, sugar alcohols, alkaloids, organic acids and trace elements, are very similar to those of natural Cordyceps sinensis, and the types of amino acids contained are also the same, but many amino acids are higher than those of natural Cordyceps sinensis. Cordyceps sinensis. Pharmacological studies have shown that Paecilomyces tenabilis has a good protective effect on hepatitis B virus, alcohol-induced liver injury, liver fibrosis in vitro, and acute liver injury from carbon tetrachloride in vivo, and the effect is strong...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A61K36/06A61P1/16C12R1/79
Inventor 陈祝安谭悠久张程艳吕雯婷孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD