Method for preparing medium-low molecular weight dextran by using biological method
A technology of dextran and low molecular weight, which is applied in the field of biological preparation of low and medium molecular weight dextran, can solve the problems of high cost and many steps, achieve the effects of reduced usage, increased yield, and omission of acid hydrolysis operations
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Embodiment 1
[0034] Strains: Leuconostoc Mesenteroides, L.M. CICC-21725 and Penicillium cyclopium CICC-4022 were purchased from China Center of Industrial Culture Collection (CICC).
[0035] 1. Preparation of Penicillium arcus seed solution
[0036] (1) Penicillium arcus seed preparation
[0037] Penicillium arcus seed culture and preservation medium preparation: seed culture and preservation medium is CM0015 Chase agar medium: 3% sucrose, 0.3% sodium nitrite, 0.05% magnesium sulfate heptahydrate, 0.05% potassium chloride, sulfuric acid tetrahydrate Ferrous iron 0.001%, dipotassium hydrogen phosphate 0.1%, agar 1.5%, pH6.0~6.5.
[0038] Weigh each medium component according to the above formula of culture and preservation medium, put it into a clean beaker, add a certain amount of distilled water, stir and heat to dissolve completely, measure its pH with precision pH test paper, and adjust the pH of the solution with acid and alkali value to 6.0~6.5; then put it into an autoclave for ste...
Embodiment 2
[0079] Strains: Leuconostoc Mesenteroides, L.M. CICC-21725 and Penicillium cyclopium CICC-4022 were purchased from China Center of Industrial Culture Collection (CICC).
[0080] 1. Preparation of Penicillium arcus enzyme solution
[0081] According to the expanded culture conditions of Example 1, the Penicillium arcuatus seed liquid after the expanded culture for 3 days was centrifuged at 8000r / min for 10 minutes, and the supernatant was taken to obtain the Penicillium arcuciformis enzyme solution, which is called B liquid for short.
[0082] 2. Preparation of dextran by fermentation
[0083] (1) Preparation of Leuconostoc enterolis seed solution
[0084] Same as Example 1.
[0085] (2) Preparation of fermentation medium
[0086] The fermentation medium for the synthesis of dextran by fermentation with Leuconostoc enterococci L.M.CICC-21725 is: 13% sucrose, 0.7% peptone, 0.2% disodium hydrogen phosphate, 0.05% potassium dihydrogen phosphate, and 0.5% yeast extract. pH 7.2-...
Embodiment 3
[0093] Strains: Leuconostoc Mesenteroides, L.M. CICC-20074 and Penicillium cyclopium CICC-4021 were purchased from China Center of Industrial Culture Collection (CICC).
[0094] The process was the same as in Example 1. As a result, the molecular weight of the dextran obtained by the dual-bacteria coupled fermentation was about 70,000, the D value of the molecular weight distribution was 1.031, and the yield was 88%.
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