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Method for preparing medium-low molecular weight dextran by using biological method

A technology of dextran and low molecular weight, which is applied in the field of biological preparation of low and medium molecular weight dextran, can solve the problems of high cost and many steps, achieve the effects of reduced usage, increased yield, and omission of acid hydrolysis operations

Active Publication Date: 2014-06-04
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of many steps and high cost in the traditional preparation of low and medium molecular weight dextran, and provide a new biological method for preparing low and medium molecular weight dextran and its products

Method used

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  • Method for preparing medium-low molecular weight dextran by using biological method
  • Method for preparing medium-low molecular weight dextran by using biological method
  • Method for preparing medium-low molecular weight dextran by using biological method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Strains: Leuconostoc Mesenteroides, L.M. CICC-21725 and Penicillium cyclopium CICC-4022 were purchased from China Center of Industrial Culture Collection (CICC).

[0035] 1. Preparation of Penicillium arcus seed solution

[0036] (1) Penicillium arcus seed preparation

[0037] Penicillium arcus seed culture and preservation medium preparation: seed culture and preservation medium is CM0015 Chase agar medium: 3% sucrose, 0.3% sodium nitrite, 0.05% magnesium sulfate heptahydrate, 0.05% potassium chloride, sulfuric acid tetrahydrate Ferrous iron 0.001%, dipotassium hydrogen phosphate 0.1%, agar 1.5%, pH6.0~6.5.

[0038] Weigh each medium component according to the above formula of culture and preservation medium, put it into a clean beaker, add a certain amount of distilled water, stir and heat to dissolve completely, measure its pH with precision pH test paper, and adjust the pH of the solution with acid and alkali value to 6.0~6.5; then put it into an autoclave for ste...

Embodiment 2

[0079] Strains: Leuconostoc Mesenteroides, L.M. CICC-21725 and Penicillium cyclopium CICC-4022 were purchased from China Center of Industrial Culture Collection (CICC).

[0080] 1. Preparation of Penicillium arcus enzyme solution

[0081] According to the expanded culture conditions of Example 1, the Penicillium arcuatus seed liquid after the expanded culture for 3 days was centrifuged at 8000r / min for 10 minutes, and the supernatant was taken to obtain the Penicillium arcuciformis enzyme solution, which is called B liquid for short.

[0082] 2. Preparation of dextran by fermentation

[0083] (1) Preparation of Leuconostoc enterolis seed solution

[0084] Same as Example 1.

[0085] (2) Preparation of fermentation medium

[0086] The fermentation medium for the synthesis of dextran by fermentation with Leuconostoc enterococci L.M.CICC-21725 is: 13% sucrose, 0.7% peptone, 0.2% disodium hydrogen phosphate, 0.05% potassium dihydrogen phosphate, and 0.5% yeast extract. pH 7.2-...

Embodiment 3

[0093] Strains: Leuconostoc Mesenteroides, L.M. CICC-20074 and Penicillium cyclopium CICC-4021 were purchased from China Center of Industrial Culture Collection (CICC).

[0094] The process was the same as in Example 1. As a result, the molecular weight of the dextran obtained by the dual-bacteria coupled fermentation was about 70,000, the D value of the molecular weight distribution was 1.031, and the yield was 88%.

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Abstract

The invention discloses a method for preparing medium-low molecular weight dextran by using a biological method. The method comprises the following steps: adding a leuconostoc mesenteroides seed solution in a fermentation medium containing sucrose for carrying out fermentation culture; then adding a penicillium cyclopium seed solution or fermentation broth supernate of the penicillium cyclopium for continuously fermenting and culturing; and separating dextran from the fermentation broth to obtain the medium-low molecular weight dextran. The invention further discloses the prepared dextran and application thereof. The invention provides a method for directly obtaining the medium-low molecular weight dextran by using the biological method in a mode of coupling fermentation of double microorganisms. The medium-low molecular weight dextran is synthesized by directionally regulating and controlling through the biological method, so that the generated dextran degrades to form dextran which tends to be required by clinical application. Meanwhile, according to the method disclosed by the invention, ethanol precipitation can be directly carried out, and thus redundant, time-consuming and noble gradual dividing precipitation is omitted, the yield of the dextran is increased, and the use amount and the consumption of ethanol are reduced.

Description

technical field [0001] The invention belongs to the field of fine chemicals, and in particular relates to a method for preparing medium and low molecular weight dextran by a biological method and its product. Background technique [0002] Dextran (dextran), also known as dextran (scientific name), is mainly an exopolysaccharide produced by the fermentation of sucrose by certain microorganisms. Dextran was first used in the field of medicine, and the most important and earliest use was as a blood volume expander, that is, instead of plasma. It is currently internationally recognized as the preferred drug for substituting plasma. Dextran with different relative molecular weights has many pharmacological activities. In addition, dextran can be hydrolyzed into compounds of lower molecular weight in the human body, and will be metabolized to produce glucose, which has a certain nutritional effect. Currently, there are three specifications commonly used clinically: Dextran 70, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/08A61K8/73A61K31/721A61K47/36A61P7/08C09K8/035A01N43/16A01P7/00C09K17/18A23L1/09C12R1/80C12R1/645
Inventor 廖安平蓝平谢涛李媚蓝丽红梁明征
Owner GUANGXI UNIV FOR NATITIES
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