Method for preparing assembly type polymer micro fluidic chip equipped with integrated pneumatic micro valve

Abstract

The present invention provides a method for preparing an assembly type polymer micro fluidic chip equipped with an integrated pneumatic micro valve, which is characterized in that the polymer chips can be assembled into a whole chip with a structure form of four layers 'plastic gas channel control substrate-PDMS...PDMS-plastic liquid channel substrate'. The gas channel control substrate and the liquid channel substrate irreversibly sealing joint one sheet of PDMS elastic membrane respectively to prepare a gas channel control semi chip and a liquid channel semi chip, and are reversibly sealed by virtue of the natural adhesive force of PDMS membranes of two semi chips to obtain the polymer whole chip with an integrated micro pneumatic micro valve or micro valve array. The method has the advantages of simple preparation technique, low cost and high success rate; and the liquid channel semi chip or the gas channel control semi chip can be assembled freely and changed independently, which is particularly suitably used as a disposable device.

Description

technical field [0001] The invention relates to a preparation method of a polymer microfluidic chip. The polymer chip integrates miniature pneumatic microvalves or microvalve arrays in the form of a four-layer structure, and its gas circuit control channel part and liquid circuit channel part can be freely assembled and replaced independently. The chip designed and prepared according to actual needs can be used as a disposable device and applied to chemical and biochemical analysis, microchemical reaction research, cell biology research, tissue engineering and other Lab on a chip (Lab on a chip) systems. Background technique [0002] The manipulation and control of microfluidics is the basis of microfluidic chips and one of the key technologies of lab-on-a-chip systems. In a microfluidic chip system, the fluid is generally driven by a pump and controlled by a valve. At present, in the microfluidic chip system, the pneumatic microvalve with elastic membrane as the actuating...

AI Technical Summary

Problems solved by technology

If a thicker (> 1 mm) hard plastic substrate is used to increase the rigidity of the entire chip, since the sealing surface cannot be closely attached by rolling, it is inevitable that the PDMS will be damaged during the sealing process. Bubbles are left on the bonding surface between the film and the hard substrate, causing the chip to fail due to air or liquid leakage

In addition, when the three-layer structure chip of "gas circuit control substrate-PDMS membrane-liquid circuit substrate" prepared by irreversible sealing is used as a disposable chip, it is uneconomical to replace a chip as a whole every time a sample is analyzed. It also adds the trouble of frequently connecting the control gas line and solution line, making the whole operation process tedious and time-consuming, and the system may leak air or liquid due to improper connection, affecting the stability of the analysis system and the reliability of the analysis results

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