Unlock instant, AI-driven research and patent intelligence for your innovation.

Modified photosensitizer based on 5-aminolaevulic acid and preparation method thereof

A technology of aminolevulinic acid and photosensitizer, which is applied in the field of photosensitizers to achieve the effect of improving killing efficiency and improving penetration

Inactive Publication Date: 2014-01-29
XI AN JIAOTONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 1995, some people have prepared 5-ALA lipid derivatives to improve its lipophilic properties and increase its permeability to cell membranes. So far, there have been many studies on this aspect, but none of the methods have satisfied people and generally accepted

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modified photosensitizer based on 5-aminolaevulic acid and preparation method thereof
  • Modified photosensitizer based on 5-aminolaevulic acid and preparation method thereof
  • Modified photosensitizer based on 5-aminolaevulic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of the modified photosensitizer based on 5-aminolevulinic acid comprises the following steps:

[0030] 1) Preparation of cationic colloidal gold:

[0031] Take 0.1g of polydiallyldimethylammonium chloride PDDA (Polydiallyldimethylammonium chloride PDDA) and place it in a 50mL beaker, stir it evenly with a glass rod, and set the volume to 100mL.

[0032] Transfer 100mL PDDA solution to a 250mL beaker, stir magnetically for 10 minutes, add 3mL chloroauric acid with a mass fraction of 1%, and stir magnetically for 10 minutes to fully mix. Add 2 mL of absolute ethanol, stir magnetically for 2 minutes, and adjust the pH value to 8.5 with sodium hydroxide solution. Finally, heat to boiling with a microwave oven, cool to room temperature and store in a 4°C refrigerator.

[0033] 2) Combination of gold nanoparticles and 5-ALA:

[0034] Three different concentrations of 5-ALA, 1mM, 10mM and 100mM, were used to adjust the pH value to about 7.4. Filter the newl...

Embodiment 2

[0038] The preparation of the modified photosensitizer based on 5-aminolevulinic acid comprises the following steps:

[0039] 1) Preparation of cationic colloidal gold:

[0040] Take 0.1g of polydiallyldimethylammonium chloride PDDA (Polydiallyldimethylammonium chloride PDDA) and place it in a 50mL beaker, stir it evenly with a glass rod, and set the volume to 100mL.

[0041] Transfer 100mL of PDDA solution to a 250mL beaker, stir magnetically for 10 minutes, add 5mL of 1% chloroauric acid, and stir magnetically for 10 minutes to fully mix. Add 2 mL of absolute ethanol, stir magnetically for 2 minutes, and adjust the pH value to 8.0 with sodium hydroxide solution. Finally, heat to boiling with a microwave oven, cool to room temperature and store in a 4°C refrigerator.

[0042] 2) Combination of gold nanoparticles and 5-ALA:

[0043] Adjust the pH value of 5-ALA with a concentration of 10mM to about 7.2, filter the newly prepared colloidal gold solution with a 220 μm syringe...

Embodiment 3

[0047] The preparation of the modified photosensitizer based on 5-aminolevulinic acid comprises the following steps:

[0048] 1) Preparation of cationic colloidal gold:

[0049] Take 0.1g of polydiallyldimethylammonium chloride PDDA (Polydiallyldimethylammonium chloride PDDA) and place it in a 50mL beaker, stir it evenly with a glass rod, and set the volume to 100mL.

[0050] Transfer 100mL of PDDA solution to a 250mL beaker, stir magnetically for 10 minutes, add 5mL of chloroauric acid with a mass fraction of 2%, and stir magnetically for 10 minutes to fully mix. Add 2 mL of absolute ethanol, stir magnetically for 2 minutes, and adjust the pH value to 8.0 with sodium hydroxide solution. Finally, heat to boiling with a microwave oven, cool to room temperature and store in a 4°C refrigerator.

[0051] 2) The combination of nano gold and 5-ALA:

[0052] Adjust the pH value of 5-ALA with a concentration of 10mM to about 7.2, filter the newly prepared colloidal gold solution wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a modified photosensitizer based on 5-aminolaevulic acid and a preparation method thereof. The modified photosensitizer is characterized by combining 5-aminolaevulic acid and cationic colloidal gold by electrostatic force in a solution with the pH value of 7.20-7.45; using penetrating force of nano colloidal gold, and combining 5-ALA and cationic nano gold (GNPs) by electrostatic attraction, thus the penetrability of 5-ALA to the cell membrane is raised, so that the efficiency of killing tumor cells in photodynamic therapy is raised. Compared with separate nano gold and 5-ALA, the combination has obviously improved killing efficiency, and the effect of inhibiting K562 cell viability of the combination is raised to about 71% from 60% of that of separate 5-ALA, thereby proving that the novel photosensitizer has good photodynamic treatment effect and application values in photodynamic therapy.

Description

technical field [0001] The photosensitizer technical field of the present invention relates to a modified photosensitizer based on 5-aminolevulinic acid. Background technique [0002] Photodynamic therapy (photodynamic therapy, PDT) is a new method for the treatment of malignant tumors developed in the past 20 years. It uses an external photosensitizer or photosensitizer precursor to increase the accumulation of photosensitizers in the tumor, and then gives specific wavelengths of excitation light to activate the photosensitizers, thereby triggering cellular oxidative stress and causing tumor cell death. Compared with traditional surgery, radiotherapy, and chemotherapy, it has the characteristics of relative selection specificity, less toxic and side effects, non-invasiveness, and no cumulative toxicity. In recent years, the health departments of the United States, Japan, the Netherlands and Canada have successively confirmed the legitimacy of this new tumor therapy. At th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/32A61K47/02A61P35/00
Inventor 姚翠萍王晶张镇西梅建生李政许澔王斯佳韩玉龙
Owner XI AN JIAOTONG UNIV