Method for determining neutral detergent fiber content in pasture

A neutral detergent fiber and measurement method technology, applied in the direction of weighing by removing certain components, can solve the problems of difficulty in ensuring timeliness, cumbersome experimental steps, expensive equipment and filter bags, etc., and the conditions for determination are easy Satisfies, simplifies the configuration process, and improves the effect of timeliness

Active Publication Date: 2012-10-17
CHINA AGRI UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

The experimental steps of this method are cumbersome, the use of chemical reagents is more, and the number of samples measured at one time is small, and a vacuum filtration device is required, which has strict requirements on the extraction time
The filter bag technology is a method jointly developed by the United States and Canada. The analysis filter bag made of special materials is used as the carrier of the measurement, and the fiber meter is used to complete the measurement process. During the measurement process, the fiber meter can automatically complete the measurement procedure, which has a high degree of automation, Large amount of sample measurement, high accuracy, etc., but the cost of equipment and filter bags is very expensive
In addition, the above-mentioned methods are all laboratory methods, which must rely on laboratory conditions to carry out testing work. The requirements for experimental conditions are relatively strict, and it is not suitable to carry out testing at the production site.

Method used

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  • Method for determining neutral detergent fiber content in pasture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the mensuration of alfalfa neutral detergent fiber content

[0043] (1) After cutting the alfalfa to 1-2 cm, place it in a dry and ventilated environment to dry naturally. The air-dried samples were ground with a food grinder to a particle size of 18 mesh.

[0044] (2) Put 1.03 g of the above-mentioned alfalfa sample and 100 ml of neutral washing liquid into a storage bottle (volume 150 ml); screw on the storage bottle cap, and tighten it properly to maintain internal and external ventilation. Then, put the bottle into the pressure cooker, add water to the neck of the pot (the place where the mouth of the bottle is convex), and put it on the fire. After boiling, adjust to a weak boiling state and keep for 10 minutes to degas. Do not cover the pressure cooker during this process.

[0045] (3) Tighten the cap of the preservation bottle, add water to the neck of the bottle, cover the lid of the pressure cooker and put it on the fire, keep the pressurized s...

Embodiment 2

[0050] Embodiment 2, the mensuration of Leymus chinensis neutral detergent fiber content

[0051] (1) After cutting the Leymus chinensis to 1-2 cm, place it in a dry and ventilated environment to dry naturally. The air-dried samples were ground with a food grinder to a particle size of 40 mesh.

[0052] (2) Put 0.97g sample of Leymus chinensis above and 100ml of neutral detergent into a preservation bottle (volume 150ml); screw on the cap of the preservation bottle, and tighten it moderately to maintain internal and external ventilation. Then, put the bottle into the pressure cooker, add water to the neck of the pot (the place where the mouth of the bottle is convex), and put it on the fire. After boiling, adjust to a weak boiling state and keep for 10 minutes to degas. Do not cover the pressure cooker during this process.

[0053] (3) Tighten the cap of the preservation bottle, add water to the neck of the bottle, cover the lid of the pressure cooker and put it on the fire, ...

Embodiment 3

[0058] Embodiment 3, the mensuration of white clover neutral detergent fiber content

[0059] (1) After cutting the white clover to 1-2 cm, place it in a dry and ventilated environment to dry naturally. The air-dried samples were ground with a food grinder to a particle size of 30 mesh.

[0060] (2) Put 1.08 g of the above-mentioned white clover sample and 100 ml of neutral washing liquid into a storage bottle (volume 150 ml); screw on the storage bottle cap, and tighten it properly to maintain internal and external ventilation. Then, put the bottle into the pressure cooker, add water to the neck of the pot (the place where the mouth of the bottle is convex), and put it on the fire. After boiling, adjust to a weak boiling state and keep for 10 minutes to degas. Do not cover the pressure cooker during this process.

[0061] (3) Tighten the cap of the preservation bottle, add water to the neck of the bottle, cover the lid of the pressure cooker and put it on the fire, keep th...

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Abstract

The present invention provides a method for determining acid detergent fiber content in pasture. The method includes the following steps: (1) mixing and placing a neutral washing liquid and the pasture with a weight of A in a preservation bottle, and placing the preservation of the bottle in a pressure cooker, and heating to boiling for degassing; (2) sealing the preservation bottle and pressurizing the bottle in the pressure cooker to obtain a mixed liquid; (3) filtering the mixed liquid obtained in the step (2) with a filter with a weight of B to obtain a filter cake namely the neutral detergent fiber; and (4) drying the filter and the filter cake to a constant weight of C and then calculating according to a formula (I) to obtain the neutral detergent fiber (NDF) content in the pasture (%). Through depth analysis of NDF measurement principle, the present invention develops a simple method for determining NDF content in pasture.

Description

technical field [0001] The invention relates to a method for determining the neutral detergent fiber content of forage grass, which belongs to the technical field of forage grass detection. Background technique [0002] Neutral detergent fiber (NDF) is one of the test indicators for detecting cell wall components or fiber components in plant feed, which is basically equivalent to the sum of hemicellulose, cellulose and lignin in feed. This index of diet is of great significance to maintain the digestive function and health of ruminants and ensure the production level of animal products. [0003] The Van Soest washing fiber method is a classic method for determining NDF. This method uses neutral washing liquid to dissolve the cell content in plant feed, and the insoluble residue is NDF. There are currently two main methods for laboratory determination: traditional suction filtration and filter bag technology that relies on imported analyzers. [0004] The suction filtration...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/04
Inventor 玉柱白春生张英俊娜日苏
Owner CHINA AGRI UNIV
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