Thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and detection method thereof

A peroxidase, homogeneous luminescence technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of inability to detect other types of antibodies, serum dilution errors, etc., and achieve fast, time-saving, high-resolution Effect

Inactive Publication Date: 2012-10-17
天津中企华科生物科技发展有限公司
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Problems solved by technology

[0009] Second: Inherent defects brought about by the indirect method response mode
There are inherent defects in this indirect detection mode, which are as follows: ① Since the enzyme-labeled antibody is only for IgG, it cannot dete

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  • Thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and detection method thereof
  • Thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and detection method thereof
  • Thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and detection method thereof

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[0038] The present invention will be described in detail below with reference to the drawings and embodiments.

[0039] 1. Detection principle

[0040] 1. Light excitation coupled immunoassay technology The light excitation coupled immunoassay method system consists of two kinds of 100 nanometer microspheres, which use the microspheres as donor and acceptor to detect the interaction of biomolecules. The surface of the microspheres is covered with a layer of hydrogel, which acts as a functional gene for biological connection. Relying on the interaction of biomolecules to bring the donor and acceptor microspheres closer together, the chemical reaction of cascade amplification is stimulated and the signal is greatly enhanced. Specifically, under the irradiation of laser light (wavelength 680 nm), the photosensitizer on the donor microspheres converts oxygen in the surrounding environment into more active monomeric oxygen. The monomer oxygen diffuses to the acceptor microspheres and ...

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Abstract

The invention discloses a thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and a detection method thereof. The thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit comprises a homogeneous-phase luminescent 96-pore determination plate, a thyroperoxidase antibody standard product, a streptavidin coated donor microsphere solution and a biotin labelled thyroperoxidase antigen, as well as a thyroperoxidase antigen coated receptor microsphere solution. The thyroperoxidase antigen in a sample is competitive with the thyroperoxidase antigen on the receptor microspheres to combine with the biotin labelled thyroperoxidase antigen. The detection method comprises the steps of measuring the luminescence signals of known standard products with different contents, drawing a standard curve and obtaining a mathematical function, and obtaining the content of unknown sample by the mathematical function. The detection method integrates the advantages of both enzyme immunoassay and chemiluminescence immunoassay, and accurately determines the level of the thyroperoxidase antibody in serum, is applied for screening, diagnosing and curative effect evaluation on high-risk groups with autoimmune thyroid disease.

Description

technical field [0001] The invention relates to a light excitation coupling immunoassay technology, in particular to a homogeneous luminescent immunoassay kit for thyroid peroxidase antibody and a detection method thereof. Background technique [0002] Thyroid peroxidase (TPO) is a membrane-bound glycoprotein containing a heme prosthetic group. The whole molecule is divided into an inner region, a transmembrane region and an outer region. active. As an important enzyme in the synthesis of thyroid hormone, TPO can catalyze the activation of iodine, the iodination of tyrosine residues on thyroglobulin and the coupling of iodinated tyrosine residues, and finally generate thyroxine (T 3 , T 4 ). When thyroid disease occurs and the follicular cell structure is destroyed, TPO leaks from the edge of the thyroid follicular cell cavity, that is, the top of the thyroid cells, to the peripheral blood, stimulating the body's immune system to produce TPO antibodies (anti-TPO antibodie...

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Application Information

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IPC IPC(8): G01N33/543G01N33/531
Inventor 李会强孙颖张明华王高升单立新苑凤君薛寒
Owner 天津中企华科生物科技发展有限公司
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