Unlock instant, AI-driven research and patent intelligence for your innovation.

Probe for detecting mpl gene polymorphism and use of the same

A technology of polymorphism detection and polymorphism, applied in genetic engineering, plant gene improvement, application, etc.

Inactive Publication Date: 2012-10-17
ARKRAY INC
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, such a detection method utilizing Tm analysis, for example, must judge a difference of one base by the Tm value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probe for detecting mpl gene polymorphism and use of the same
  • Probe for detecting mpl gene polymorphism and use of the same
  • Probe for detecting mpl gene polymorphism and use of the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] In this example, under the coexistence of wild-type and mutant plasmids, Tm analysis was performed to detect the polymorphism of the MPL gene.

[0126]Wild-type plasmid (wt), mutant-type plasmid (W515L) and mutant-type plasmid (W515K) were produced. The above wt is made into a partial sequence of the wild-type MPL gene in which the base w of the base number 11534 of the sequence number 1 is thymine (t), and the base d of the base number 11535 is guanine (g). 1 base number 11391-11711) double-stranded plasmid. The above-mentioned W515L was prepared as a double-stranded plasmid in which a partial sequence of the W515L mutant MPL gene (base numbers 11391-11711 of SEQ ID NO: 1) in which the base d of the above-mentioned base number 11535 was mutated to thymine (t) was inserted. The above-mentioned W515K was made into a partial sequence of the W515K mutant MPL gene in which the base w of the above-mentioned base number 11534 was mutated to adenine (a) and the base d of the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided are a probe for detecting polymorphism and a method for using the same with which MPL gene polymorphism can be readily identified with excellent reliability. A probe comprising at least one of the oligonucleotides (P1), (P1'), (P2), and (P2') serves as the probe for detecting MPL gene polymorphism. (P1) An oligonucleotide that has a base length of 9 to 50 bases, is formed from a base sequence that includes bases No. 11535 through 11543 in Sequence No. 1, and has base No. 11543 in the 3' terminal region (P1') An oligonucleotide formed from a base sequence complementary to the oligonucleotide (P1) (P2) An oligonucleotide that has a base length of 10 to 50 bases, is formed from a base sequence that includes bases No. 11535 through 11544 in Sequence No. 1, and has base No. 11544 in the 3' terminal region (P2') An oligonucleotide formed from a base sequence complementary to the oligonucleotide (P2).

Description

technical field [0001] The present invention relates to a probe for detecting polymorphism of MPL gene and use thereof. Background technique [0002] Chronic myeloproliferative disease (MPD) is a clonal disease caused by abnormalities in hematopoietic stem cells, including chronic myelogenous leukemia (CML), polycythemia vera (PV), essential thrombocythemia (ET), primary Generic term for diseases such as myelofibrosis (PMF). It is known that in some patients with essential thrombocythemia and primary myelofibrosis, due to mutations in the MPL (myeloproliferative leukemia) gene encoding the thrombopoietin receptor, tryptophan at position 515 of the MPL protein The acid (W) is substituted with leucine (L) or lysine (K) (Non-Patent Document 1). The above-mentioned substitution (W515L) known to be substituted with leucine (L) is a mutation of base guanine (g) at base number 11535 in the base sequence of the MPL gene shown in SEQ ID NO: 1 to thymine (t), and the above-mentione...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/00C12N15/09C12Q1/68
CPCC12Q2600/156C12Q1/6883C12N15/11
Inventor 平井光春小森真理子
Owner ARKRAY INC