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Breeding method of multi-resistance rice germplasm

A rice, multi-resistance technology, applied in the biological field to achieve the effect of improving breeding efficiency and shortening breeding time

Active Publication Date: 2012-10-31
FUJIAN HEFENG SEED IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been a few research reports on the introduction of 2-3 disease resistance genes into rice by marker-assisted selection technology, but they are all aggregated for resistance genes scattered on different chromosome segments, and no rice disease resistance genes have been found. A report on the creation of multi-resistance rice germplasm based on the characteristics of "cluster distribution"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Carry bacterial blight resistance gene Xa21 and Xa23 The donor parent L17 (Ni Dahu from the Rice Research Institute of Anhui Academy of Agricultural Sciences, etc. Pi9 (t) gene and bacterial blight resistance gene Xa21 and Xa23 strains) (Ni Dahu et al. Molecular marker-assisted breeding of rice three-gene aggregation lines resistant to bacterial blight and rice blast. Acta Crops Sinica. 2008, 34(1): 100-105) and carrying the rice blast resistance gene Pi-K h and Pi-1 MA119-2-3 in the 07 (Jiang Yunlin of Fujian Agriculture and Forestry University selected by molecular marker-assisted breeding technology to carry Pi-K h and Pi-1 Gene II-32B disease-resistant near-isogenic line) (Jiang Yunlin. A study on the breeding of long-lasting disease-resistant hybrid rice parents using MAS technology. Master's degree thesis of Fujian Agriculture and Forestry University, 2008, p. 63) hybridization, obtained F 1 .

[0021] 2. Plant F 1 , harvest seeds when mature, ...

Embodiment 2

[0033] 1. Carry bacterial blight resistance gene Xa21 and Xa23 The new line R106 (bred by Luo Yanchang et al. Xa21 and Xa23 Gene new strains) (Luo Yanchang et al. Research on the breeding of a two-gene three-line sterile line R106A resistant to bacterial blight of rice. Chinese Agricultural Sciences, 2005, 38 (11): 2157-2164) and simultaneously carrying rice blast resistance gene Pi-Kh and Pi-1 MA120-2-2 in the 07 (Jiang Yunlin of Fujian Agriculture and Forestry University selected by molecular marker-assisted breeding technology to carry Pi-K h and Pi-1 Ⅱ-32B disease-resistant near-isogenic line of Ⅱ-32B disease-resistant near-isogenic line) (Jiang Yunlin. A study on the breeding of durable disease-resistant hybrid rice parents using MAS technology. Master's degree thesis of Fujian Agriculture and Forestry University, 2008, No. 63 p) crossed to obtain F 1 .

[0034] 2. Plant F 1 , harvest seeds when mature, get F 2 seed.

[0035] 3. Plant F 2 , at F 2 In ...

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Abstract

The invention relates to a breeding method of multi-resistance rice germplasm, belonging to the technical field of biology. The breeding method is as follows: a parent P1 simultaneously carrying anti-xanthomonas oryzae gene Xa21 and Xa23 and a parent P2 simultaneously carrying anti-rice blast disease gene Pi-Kh and Pi-1 as parent raw materials, and Pi1, PiKh, Xa21 and Xa23 dispersed in different resistant sources are guided into the same rice germplasm by convergent cross and molecular marker-assisted selection technology, so that the new rice germplasm carrying multi-resistance-section is created, and the resistance of the parent of good hybrid rice can be improved rapidly by utilizing the multi-resistance germplasm. The germplasm can be directly used for preparing anti-disease hybrid rice combination, and also can be taken as the resistant source of the parent of a new circle of anti-disease hybrid rice. More importantly, a plurality of multi-disease genes can be guided into the same one breeding group by one-time hybridization of the multi-resistance germplasm, so that the breeding time is shortened greatly, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to a method for breeding multi-resistant rice germplasm, which belongs to the field of biotechnology. Background technique [0002] Rice plays an important role in food production, but the problem of disease resistance of rice is still very prominent. Most of the approved varieties or combinations are not strong resistant, and few varieties are resistant to two major diseases at the same time. The main reasons for the above situation are as follows: 1. At present, most breeding units adopt traditional breeding methods for disease resistance breeding, and the breeding efficiency is low; 2. There is a lack of resistance sources with excellent comprehensive traits, and the breeding period is long; 3. The lack of simultaneous carrying There are germplasms with multiple known disease resistance genes, and the breeding efficiency is low. Therefore, creating rice parents carrying multiple disease-resistant genes is of great significance...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 陈志伟官华忠吴为人周元昌潘润森毛大梅
Owner FUJIAN HEFENG SEED IND CO LTD
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