Kit for detecting or supplementarily detecting medullary leukemia cell differentiation stages

A technology for myeloid leukemia and leukemia cells, applied in the field of kits for detecting or assisting in the detection of the differentiation stage of myeloid leukemia cells

Active Publication Date: 2012-11-07
武汉康圣达医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although a variety of immune markers, molecular markers or genetic changes can be used to distinguish AML subtypes with different clinical features, most AML still have no clear molecular markers to distinguish them and are classified as The untyped group can only be roughly divided into approximate differentiation stages with reference to the FAB type

Method used

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  • Kit for detecting or supplementarily detecting medullary leukemia cell differentiation stages
  • Kit for detecting or supplementarily detecting medullary leukemia cell differentiation stages
  • Kit for detecting or supplementarily detecting medullary leukemia cell differentiation stages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1. Analysis of VSTM1 expression in different myeloid cells

[0074] 1. Detection of VSTM1 expression in normal bone marrow cell differentiation stage

[0075] 1. Expression of VSTM1 in various cell subsets of normal bone marrow

[0076] In order to clarify the expression level of VSTM1 protein in each subgroup of bone marrow cells, different fluorescein-labeled antibodies against leukocyte surface antigen and anti-VSTM1 antibody were used to mark bone marrow cells, and flow cytometry was used to detect the expression level of VSTM1 in 29 normal donor bone marrow cells. Expression in cell subpopulations.

[0077] Using CD45 and side scatter light (SSC) to divide cells into lymphocytes (CD45++ and small SSC), immature cells (CD45+ and small SSC), mature granulocytes (CD45+ and large SSC) and monocytes (CD45++ and small SSC) ).

[0078] The above-mentioned flow cytometry was used to detect the expression of VSTM1 in the bone marrow cell subsets of 29 normal dono...

Embodiment 2

[0096] Example 2, the kit for detecting the differentiation stage of myeloid cells and its application

[0097] 1. Expression of VSTM1 in bone marrow cells of different differentiation stages of AML and construction of the kit

[0098] AML is usually classified according to the FAB (French-American-British) classification, which is based on different types of bone marrow cells and their degree of differentiation. 52 cases of AML acute myeloid leukemia (4 cases of M0 / M1, 22 cases of M2, 4 cases of M3, 14 cases of M4, 8 cases of M5, the FAB type of each patient has been determined), in order to further understand the expression of VSTM1 and the different differentiation of AML To analyze the relationship between bone marrow cells of different stages, the expression of VSTM1 in AML bone marrow cells of different FAB subtypes was analyzed. Normal human bone marrow cells were used as controls.

[0099] The detection method uses the above-mentioned flow cytometry to detect the exp...

Embodiment 3

[0118] Example 3, the relationship between the expression of VSTM1 in AML bone marrow cells and CD34 and CD33, and the induction of differentiation of M3 subtype bone marrow cells

[0119] 1. The relationship between the expression of VSTM1 in AML bone marrow cells and CD34 and CD33

[0120] The Gene Atlas database indicated that VSTM1 was selectively highly expressed in bone marrow CD33+ myeloid cells, while the expression abundance in other tissues and cells was very low. Moreover, VSTM1, like CD33, has an intracytoplasmic tail ITIM motif. Therefore, the expression of VSTM1 in myeloid cells and its relationship with the expression of CD33 were analyzed.

[0121] The flow cytometry software was used to set gates and analyze different cell subpopulations. The detection method was the above-mentioned flow cytometry detection, and the antibodies were VSTM1-FITC, CD45-PerCP, CD33-APC, and CD34-PE.

[0122] The result is as Figure 8 As shown in Table 2, A: Example of VSTM1 exp...

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Abstract

The invention discloses a kit for detecting or supplementarily detecting medullary leukemia cell differentiation stages. The kit comprises a device for detecting VSTM1 (V-set and transmembrane domain-containing 1) protein expression and a reagent for detecting the VSTM1 protein expression. The amino acid sequence of the VSTM1 protein is a sequence 1 in a sequence table. The experiment in the invention shows that VSTM1 protein expression is associated with differentiation of medullary cells. Therefore, a kit which can be used for detecting acute medullary leukemia cell differentiation stages is obtained. The kit is used to detect the expression quantity of VSTM1 protein expression, so that the different differentiation stages of the acute medullary leukemia cell differentiation stages is judged, and the differentiation process of the acute medullary leukemia can be early reflected in detail, thereby exerting an important effect in diagnosis and treatment of leukemia.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting or assisting in detecting the differentiation stage of myeloid leukemia cells. Background technique [0002] Leukemia is a type of malignant disease characterized by hematopoietic cell differentiation block. It is currently divided into lymphocytic leukemia, myeloid leukemia, erythroleukemia, and megakaryoblastic leukemia according to the type of cells involved. Among them, acute myeloid leukemia (AML) presents great heterogeneity due to different stages of myeloid cell differentiation arrest. The traditional FAB classification of AML is not enough to reflect the characteristics of AML, and its clinical application is limited. [0003] Therefore, the currently widely used WHO classification incorporates the expression of immune markers (immunotyping), cytogenetic changes, and molecular biology (fusion genes, mutations) of AML into classification factors, reflecti...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 黄晓军阮国瑞谢敏韩文玲马大龙陈珊珊刘艳荣李婷冷馨王绪华
Owner 武汉康圣达医学检验所有限公司
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