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A new application technology of scrophginic acid, which is applied in the field of traditional Chinese medicine, and can solve problems such as no research reports on the prevention and treatment of myocardial ischemia activity of scrophularic acid
Active Publication Date: 2012-11-14
SHANGHAI UNIV OF T C M
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Problems solved by technology
[0020] But so far, there is no research report on the prevention and treatment of myocardial ischemia with scrophginic acid.
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Embodiment 1
[0045] Example 1: In vitro experiment of the protective effect of Scrophulariaceae on cardiomyocytes
[0046] 1) H9c2 cell culture
[0047] H9c2 cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS), 100U / mL penicillin and 100μg / mL streptomycin, and placed at 37℃, 5% CO 2 Cultured in the incubator for 2 to 3 days, when the cell mass reaches 70% to 80% of the culture dish, digest with 0.125% trypsin.
[0048] 2) Determination of myocardial cell viability
[0049] This experiment uses 100μM H 2 O 2 To evaluate the protective effect of Scrophulariae red acid on oxidative stress damage of cardiomyocytes (H 2 O 2 The cytotoxicity of the concentration in the range of 0.1~1.0mmol / L is dose-dependent), the cardiomyocyte suspension was seeded in 96-well plates at 8000 / well, after 48 hours of culture, the serum-free culture medium was replaced and the culture was continued for 12 hours, then The cultured cardiomyocytes were randomly divided into normal control groups (witho...
Embodiment 2
[0056] Example 2: In vivo experiment of the protective effect of Scrophulariaceae red acid on cardiomyocytes
[0057] 1) Experimental materials
[0058] Wistar rat: male, clean grade, aged 3 to 4 weeks, weighing 200±20g, provided by the Animal Experiment Center of Shanghai University of Traditional Chinese Medicine.
[0061] Acute myocardial infarction caused by ligation of the left anterior descending coronary artery is the most commonly used method for modeling acute myocardial ischemic infarction in rats, and it is also a recognized model for studying myocardial ischemic injury. The specific modeling method is as follows: anesthetize the rat with 3% sodium pentobarbital, fix it in the supine position, shear the chest, and disinfect the skin with alcohol. Use a tracheal intubation, connect an animal respirator, and place a body surface ECG for monitoring. Make a long...
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Abstract
The invention discloses a new application of azafrin, and the new application means using the azafrin or pharmaceutically acceptable salt or ester as an active component to prepare medicines or health-care foods for preventing or/and treating a cardiovascular disease. The experimental results show that the azafrin is capable of obviously alleviating a myocardial injury caused by H2O2 (Hydrogen Peroxide) and being free of injury and toxicity on myocardial cells; and the azafrin is capable of significantly improving the function of cardiac contractility while myocardial ischemia happens, maintaining the heart rate of acute myocardial infarction after being molded, increasing the activity of serum SOD (Super Oxygen Dehydrogenises), inhibiting MDA (Methane Dicarboxylic Aldehyde) and TnI (Troponin), increasing the coronary flow, and maintaining an oxygen supply for myocardium, thereby maintaining a normal heart rate of the heart and improving the myocardial function; therefore, the azafrintaken as the active component is expected to be developed to be the medicines for preventing or/and treating the cardiovascular disease, in particular to the cardiovascular disease relative to myocardial ischemia, thereby being important for a research and an application of the azafrin.
Description
Technical field [0001] The present invention relates to a new use of Azafrin red acid (Azafrin), in particular to a new use of Azafrin red acid for preparing medicines or health foods for preventing or / and treating cardiovascular diseases, and belongs to the technical field of traditional Chinese medicine. Background technique [0002] Myocardial ischemia refers to a pathological state in which the blood perfusion of the heart is reduced, which leads to a decrease in oxygen supply to the heart, and abnormal energy metabolism of the myocardium, which cannot support the normal work of the heart. Coronary artery stenosis or occlusion caused by coronary atherosclerosis is the main and most common cause of myocardial ischemia, which in turn leads to myocardial ischemia and hypoxia. The resulting heart disease is what we often call “coronary heart disease”. Coronary heart disease is the "culprit" of myocardial ischemia. Myocardial ischemia is a common and frequently-occurring disease ...
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