MicroRNA (micro ribonucleic acid) for regulating gene expression of B7-H4 (a molecule of the B7 family)
A B7-H4 and gene expression technology, applied in the field of regulating B7-H4 gene expression and microRNA, can solve the problems of miRNAs that are difficult, have different functions, and there is no correlation between miR-143 and B7-H4 genes.
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[0028] The above solution will be further described below in conjunction with specific embodiments. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.
[0029] 1. Reagents and materials
[0030] 1. Reagents
[0031] Fetal bovine serum (Hyclone, USA); complete medium: add 100ml of fetal bovine serum, 0.15g of L-glutamine, 10.0ml of 2-mercaptoethanol (5×10 -3 mol / L); liposome 2000 (Invitrogen, USA); penicillin, streptomycin (Shanghai Sangon Biotechnology Co., Ltd); TRIzol (Invitrogen, USA); agarose (LP0028A, Oxoid Ltd, UK); gel Electrophoresis sample solution and ethidium bromide (EtBr) (Shanghai Sangon Biotechnology Co., Ltd.); gel recovery DNA kit an...
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