Method for rapid identification of sinonovacula constricta population by utilizing mitochondrial molecular marker

A molecular marker and mitochondrial technology, which is applied in the field of identifying constricted razor clams by using mitochondrial molecular markers, can solve the problems of mixed seedlings and indigenous seedlings, difficult identification, and easy to be affected by environmental factors, and achieves strong reliability and identification methods. simple effect

Inactive Publication Date: 2012-11-14
SHANGHAI OCEAN UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The introduction and cultivation of off-site seedlings can easily lead to the mixing of off-site seedlings and indigenous seedlings. Therefore, how to distinguish seedlings from different regions for later seed selection, germplasm resource protectio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapid identification of sinonovacula constricta population by utilizing mitochondrial molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1: Using the method of the present invention, the Jianghu population and Zhejiang Min population of Sinonovacula constricta were distinguished.

[0025] (1) Collect individuals of Sinonovacula constricta along the beaches of Jiangsu, Shanghai, Zhejiang and Fujian, and extract DNA;

[0026] Individuals of Sinonovacula constricta were randomly collected from coastal beaches of Jiangsu, Shanghai, Zhejiang and Fujian in my country. Using a scalpel and forceps, the mantle tissues of the individual constriction razor clams in the four regions were collected and stored in absolute ethanol for later use.

[0027] The phenol-chloroform extraction method is used for DNA extraction, and the specific method is as follows. After cutting 0.5g of mantle tissue from each sample, add 500μL of tissue homogenization buffer (10mmol / dm3 Tris-Hcl, PH=8.0; 50mmol / dm3 EDTA, PH=8.0), mix well and add the final concentration to 1 % SDS and 200μg / cm3 proteinase K, digested and clarified at 55°...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for rapid identification of a sinonovacula constricta population by utilizing a mitochondrial molecular marker. The method comprises the following steps of: (1) collecting sinonovacula constricta individuals and extracting DNA (deoxyribonucleic acid); (2) synthesizing a mitochondrial cytochrome oxidase I marker primer; (3) performing PCR (polymerase chain reaction) amplification on genes of the mitochondrial cytochrome oxidase I (COI) of the sinonovacula constricta population; (4) purifying a PCR product; and (5) performing sequencing and sequence alignment on the purified PCR product. The method has the advantages of stable result, strong repeatability and identification simplicity.

Description

technical field [0001] The invention relates to germplasm identification of razor clams, in particular to a method for identifying populations of razor clams by using mitochondrial molecular markers. Background technique [0002] The razor clam is one of the four marine aquaculture shellfish in my country. It has the characteristics of fast growth, short breeding cycle, easy management, high yield and good benefit. According to statistics, the aquaculture output of shellfish in my country accounts for 82% of the total output of mariculture. The annual output of tidal flat shellfish is nearly 2 million tons, of which the output of razor clams has reached 700,000 tons, accounting for about 30% of the total output of tidal flat shellfish aquaculture in China, and occupies an important position in my country's mariculture. [0003] For example, the coastal areas of Zhejiang and Fujian in my country are important seed production areas of razor clams, which are transported to oth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 牛东红李家乐沈和定王劦金凯
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap