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Organic carbon mineralized culture method for determining soil organic carbon mineralized rate by utilizing apparatus without soil

A technology of organic carbon and soil, applied in soil material testing, material inspection products, etc., can solve problems such as difficult continuous sampling and experimental errors of cultivation devices, and achieve the effect of small experimental errors, high accuracy and precision

Inactive Publication Date: 2014-09-03
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention solves the shortcomings of the existing lye absorption method, gas chromatography method and infrared gas analyzer method that the experimental error of the cultivation device is more than 10%, and it is difficult to continuously sample, and only measures CO for the above test methods. 2 , not measured for CH 4 problem, and provides a soilless method for determining the mineralization rate of soil organic carbon

Method used

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  • Organic carbon mineralized culture method for determining soil organic carbon mineralized rate by utilizing apparatus without soil
  • Organic carbon mineralized culture method for determining soil organic carbon mineralized rate by utilizing apparatus without soil
  • Organic carbon mineralized culture method for determining soil organic carbon mineralized rate by utilizing apparatus without soil

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specific Embodiment approach 1

[0019] Specific implementation mode one: (see attached figure 1 ) The organic carbon mineralization cultivation device of this embodiment is composed of a biochemical cultivation pin 1, a valve 2, a rubber stopper 3, a connecting pipe 4, and a jar 5; wherein in the organic carbon mineralization cultivation device, the connecting pipe 4 penetrates the rubber stopper 3. The end of the connecting pipe 4 located at the larger side of the bottom of the rubber stopper 3 is connected to the biochemical culture pin 1 through the valve 2, and the rubber stopper 3 is covered on the mouth of the jar 5.

specific Embodiment approach 2

[0020] Specific implementation mode two: the present embodiment utilizes the method for soilless measurement of soil organic carbon mineralization rate by the organic carbon mineralization cultivation device, specifically completes according to the following steps:

[0021] 1. Take 2 sets of organic carbon mineralization cultivation devices A and B;

[0022] 2. Determination of soil moisture content. According to the moisture content, the volume ratio of dry soil mass to distilled water is 1g: 4.5 ~ 5.5mL. Weigh the soil and distilled water, add the weighed soil and distilled water into the beaker and stir it magnetically for 30 minutes, and then use 2900 After centrifuging at a speed of ~3100rpm for 5 minutes, take 5% of the supernatant by volume as the microbial inoculum; filter the remaining supernatant through a 0.45 μm filter membrane to obtain a water-soluble organic carbon mother liquor;

[0023] 3. Measure the microbial inoculum and water-soluble organic carbon mother ...

specific Embodiment approach 3

[0028] Embodiment 3: This embodiment is different from Embodiment 2 in that: in step 2, the soil and distilled water are weighed according to the volume ratio of dry soil mass to distilled water is 1g: 4.8-5.2mL. Others are the same as in the second embodiment.

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Abstract

The invention discloses an organic carbon mineralized culture apparatus and a method for determining soil organic carbon mineralized rate by utilizing the apparatus without soil, and in particular relates to the culture apparatus and the method of determining soil organic carbon mineralized rate by utilizing the apparatus. According to the invention, the apparatus and the mehtod aim to solve the problems that the experimental error of culture apparatus is large, continuous sampling is difficult, and the test method only determines CO2 and does not determine CH4 in the existing alkali liquor absorption method, gas chromatography and infrared gas analyzer method. The culture apparatus consists of a biochemistry culture pin, a valve, a rubber stopper, a connecting pipe and a wild-mouth bottle. The method comprises the steps of taking the apparatus; preparing and collecting microorganism inoculation liquid and water-soluble organic carbon mother liquor; preparing a culture solution; assembling a sample device; assembling a blank device; cultivating; and determining. The experimental error of the apparatus provided by the invention is decreased to less than 5 percent; continuous sampling can be carried out. With the adoption of the method provided by the invention, rates of discharge of CO2 and CO4 can be determined at the same time. The apparatus and the method can be used to determine the soil organic carbon mineralized rate.

Description

technical field [0001] A cultivation device and a method for determining soil organic carbon mineralization rate using the device. Background technique [0002] Soil stores up to 1500 Pg of organic carbon, which is 3 times the atmospheric carbon pool and 2.5 times the terrestrial biomass. Small changes in the soil organic carbon pool can lead to atmospheric CO 2 The drastic fluctuation of the concentration plays an extremely important role in the global carbon cycle. The mineralization of soil organic carbon is not only affected by temperature conditions, water conditions, soil properties and other factors, but also related to soil carbon and nitrogen content. Under the trend of global warming, how to prepare for the determination of the mineralization rate and amount of soil organic carbon has become one of the key and scientific issues of soil and global change research in recent years. [0003] Many studies at home and abroad have shown that in addition to cultivating s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/24
Inventor 邹元春于晓菲霍莉莉吕宪国姜明佟守正娄晓楠
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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