Application of Lactobacillus johnsonii in improving production performance of laying chickens

A kind of Lactobacillus johnsonii, production performance technology, applied in biological field

Inactive Publication Date: 2012-11-21
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no product in China that uses Lactobacillus johnsonii as an additive to add food and feed additives, and its application in animal and poultry breeding is rarely reported. Lactobacillus johnsonii is developed into an animal microecological feed additive. Filling in the domestic gap and screening Lactobacillus johnsonii with excellent probiotic properties is of great significance to promoting the level of livestock and poultry breeding in my country and ensuring food security in my country, and can play a greater role in livestock and poultry breeding

Method used

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  • Application of Lactobacillus johnsonii in improving production performance of laying chickens
  • Application of Lactobacillus johnsonii in improving production performance of laying chickens
  • Application of Lactobacillus johnsonii in improving production performance of laying chickens

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Embodiment 1

[0033] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. The preparation of embodiment 1 Lactobacillus johnsonii CGMCC No.4926 freeze-dried agent

[0034] 1) Activate cryopreserved Lactobacillus johnsonii CGMCC No.4926 on the MRS agar plate for three times, pick a single colony and inoculate it into a 10mL deep layer MRS liquid test tube, culture it at 37°C for 10 hours, then transfer it to 250mL MRS with 1% inoculum Liquid medium, used as seed liquid after culturing for 10 hours;

[0035] 2) Inoculate the seed solution obtained in step 1) in a 5L fermenter with an inoculation amount of 5%;

[0036] 3) During the fermentation process, the pH value of the fermentation broth is maintained at 6.0. When the pH exceeds the set value, the feed liquid is added; when the pH is lower than the set value, the alkaline neutralizer ammonia water is automatically added; Ferment at 100rpm for 14 hours, fee...

Embodiment 2

[0041] (1) Experimental animals and experimental design

[0042] 540 1-day-old female healthy Jinghong egg chicks were purchased from the hatchery of Beijing Huadu Yukou Poultry Industry Co., Ltd., and randomly divided into 6 treatment groups, each treatment group had 6 replicates, and each replicate had 15 chickens. The first group is the blank control group, fed with basic diet; the second to sixth groups are Lactobacillus johnsonii bacterial powder test group, respectively added 1.0×10 4 , 1.0×10 5 , 1.0×10 6 , 1.0×10 7 , 1.0×10 8 cfu / g of Lactobacillus johnsonii powder. The test period is 120 days. The basal diet formula is shown in Table 1. Prepared with reference to NRC (1994). It is divided into two stages: 0-6 weeks old and 7-17 weeks old.

[0043] Table 1 Basic diet formula and nutritional components

[0044]

[0045]

[0046] (2) Animal experiment methods

[0047] Adopt stacked cages, 15 / cage. Provide warm water for drinking after entering the chicks...

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Abstract

The invention discloses an application of Lactobacillus johnsonii CGMCC No. 4926 in improving production performance of laying chickens. The Lactobacillus johnsonii is added into feed in a dose of 1.0*104-108cfu/g, and accordingly feed conversion rate is improved and feed growth ratio of the laying chickens is lowered evidently.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a Lactobacillus johnsonii strain CGMCC 4926 in improving the production performance of laying chicks. Background technique [0002] Lactobacillus johnsonii belongs to the phylum Firmicutes, the class Bacilli, the order Lactobacillales, the family Lactobacillaceae, and the genus Lactobacillus. It was first isolated in 1980. It has been classified as Lactobacillus acidophilus in science. In 1992, Fujisawa et al. named it Lactobacillus johnsonii through biochemical experiments and DNA hybridization experiments. Lactobacillus johnsoni mainly exists in the digestive tract of animals and humans, and is the dominant flora. [0003] The European Food Safety Authority (EFSA) issued three health declarations against Lactobacillus johnsonii in 2009 and 2011 respectively, stating that Lactobacillus johnsonii has microorganisms that reduce intestinal pathogens, en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16C12N1/20C12R1/225
Inventor 张军汪攀孙晓雯闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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