Method for quickly and stably picking fragile tissue of animal for experiments

Abstract

The invention relates to a method for quickly and stably picking a fragile tissue of an animal for experiments, composed of a rectangular plastic vessel and a sepharose gel female die for the fragile tissue of the animal for experiments. Specifically, a female die formed of sepharose gel is arranged in a plastic container, and capable of fixing the fragile tissues such as brains of experimental animals. The size and height of the plastic container can be selected according to the size of the tissue picked for experiments. Transparent sepharose gel is convenient for observing the picked tissue structure in each direction. A device for quickly and stably picking the fragile tissue of the animal is capable of performing precooling at 4 DEG C, and put on ice for operation, thereby preventing the protein or RNA in the tissue from being degraded. The device is capable of conveniently and completely cutting off the picked tissue, and the hardness of the sepharose gel can be adjusted according to experimental material picking requirements. A plaster male die for the fragile tissue of the animal for experiments can be used repeatedly. The sepharose gel can be prepared at different solution proportions according to the specific experimental purpose, such as PBS buffer solution containing a protease inhibitor, DEPC (Diethylpyrocarbonate) water having inhibition effect on ribozyme. The method provided by the invention is simple in operation, easy to control and low in cost; and the tissue picked is disposable in experiments.

Description

technical field [0001] The invention relates to a method for rapidly and stably sampling fragile animal tissues for experiments. Background technique [0002] In the process of animal experiments, it is a commonly used experimental technique to cut out the fragile tissues of animals required for experiments, such as brain tissues, under the naked eye. At present, the method commonly used by experimenters is to place the peeled off mouse brain on a plastic container on an ice box and cut it directly. Since the mouse brain is soft and there is no obstacle around it, it is easy to cause injury when cutting the brain tissue. The mouse brain swims and collapses, which is time-consuming and labor-intensive, which affects the quality of the tissue obtained. Contents of the invention [0003] The purpose of the present invention is to provide a method for rapidly and stably obtaining materials from experimental animal fragile tissues with convenient operation and good shape. [...

AI Technical Summary

Problems solved by technology

At present, the method commonly used by experimenters is to place the peeled off mouse brain on a plastic container on an ice box and cut it directly. Since the mouse brain is soft, there is no obstacle around it and it cannot be fixed, and it is easy to cause injury when cutting the brain tissue. The mouse brain swims, collapses, etc., which is time-consuming and labor-intensive, which affects the quality of the tissue obtained

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