Method for scale-up culture of animal cells

An animal cell and cell culture technology, applied in animal cells, vertebrate cells, artificial cell constructs, etc., can solve the problems of low cell expansion and culture efficiency, inconsistent cell growth conditions, and large space occupation, and achieve cell expansion and culture efficiency. High, reduce uneven cell growth, save production space
CN102807964AInactive Publication Date: 2012-12-05BEIJING SKYWING TECH CO LTD
0 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
BEIJING SKYWING TECH CO LTD
Publication Date
2012-12-05
Estimated Expiration
Not applicable Β· inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention relates to a method for scale-up culture of animal cells. The method includes the following steps of firstly, culturing animal cells in a cell factory; secondly, digesting the cultured animal cells, namely removing culture solution, and washing the cultured animal cells by pH7.4-8.0 washing solution; removing the washing solution by adding pH7.4-8.0 digestive solution 20-80mL per layer, allowing the digestive solution to evenly flow to each layer to digest the cells at 36-38 DEG C, removing partial digestive solution when more than 60% of cells fall off from the cell culture surface, and continuing to digest by the residual 10-35% of digestive solution; and adding fresh cell culture solution containing serum or digestive solution inhibitor to finish digesting and performing cell suspension again, and adding the culture solution for once to three times repeatedly; and thirdly, inoculating obtained cell suspension to a bioreactor for culture.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for amplified cultivation of animal cells, in particular to a method for amplified cultivation of animal cells with a cell factory. Background technique

[0002] Large-scale animal cell culture technology refers to the technology of cultivating animal cells at high density and in large quantities in cell culture bioreactors under artificial conditions (setting pH, temperature, dissolved oxygen, etc.) for the production of biological products. It is widely used in the research and development and industrial production of biological products such as antibodies and virus vaccines, and is the core technology in the production of biological products.

[0003] In the actual production process, before large-scale cultivation of animal cells to produce target biological products, the number of cells that meet the production requirements must first be obtained by expanding the seed cells. The commonly used amplification method...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More