Preparation method for culture medium product for medicine industry environment microorganism monitoring

Abstract

The present invention discloses a preparation method for a culture medium product for medicine industry environment microorganism monitoring. The preparation method comprises the following steps: 1) carrying out sterilization on a culture dish to obtain a sterilized culture dish to provide a spare use; 2) melting a soybean casein agar culture medium to obtain a liquid soybean casein agar culture medium; 3) under conditions of a 10000-class cleanness background and local 10000000-class cleanness, pouring the liquid soybean casein agar culture medium into the sterilized culture dish, and cooling for 15-30 min at a room temperature to obtain a soybean casein agar culture medium dish; 4) filling a plurality of the soybean casein agar culture medium dishes in a high density polyethylene plastic bag, and sealing to obtain the packaged soybean casein agar culture medium dishes; and 5) carrying out an irradiation treatment on the packaged soybean casein agar culture medium dishes with 60 cobalt gamma-ray to obtain the culture medium product, wherein an irradiation dose is 10 Kgy. The culture medium product prepared by using the technical scheme of the present invention has the following advantages that: use is convenient and economic, and recovery rate meets requirements.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a preparation method of a culture medium product used for the monitoring of microbes in the environment of the medicine industry. Background technique [0002] The pharmaceutical industry environment usually requires microbial monitoring, including sedimentation bacteria, planktonic bacteria and surface microorganisms, generally soybean casein agar medium, the conventional preparation method and the way of transporting to the production workshop are susceptible to microbial contamination, once contaminated It is difficult to determine the source of microorganisms. Conventional preparation methods take a long time from preparation to use, which brings great inconvenience to microbial environmental monitoring work and leads to low work efficiency. There are corresponding disposable plate media on the market, but the purchase cost is high, which greatly increases the operating c...

AI Technical Summary

Problems solved by technology

[0002] The pharmaceutical industry environment usually requires microbial monitoring, including sedimentation bacteria, planktonic bacteria and surface microorganisms, generally soybean casein agar medium, the conventional preparation method and the way of transporting to the production workshop are susceptible to microbial contamination, once contaminated Difficult to determine source of microbes

Conventional preparation methods take a long time from preparation to use, which brings great inconvenience to microbial environmental monitoring work and leads to low work efficiency

There are corresponding disposable plate media on the market, but the purchase cost is high, and the operating cost of the enterprise will be greatly increased when it needs to be used in large quantities.

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