Fluorescence quantitative polymerase chain reaction (PCR) detection kit for hepatitis B virus (HBV)

Abstract

The invention relates to a fluorescence quantitative PCR detection kit for HBV. The kit comprises a PCR master mix, a fluorescence quantitative reaction solution and a positive control sample. The fluorescence quantitative reaction solution refers to a reaction system comprising a forward primer, a reverse primer and a locked nucleotide probe which are used for detecting HBV DNA, wherein the sequence of the forward primer is SEQ ID NO:1, the sequence of the reverse primer is SEQ ID NO:2, the sequence of the Taqman probe is SEQ ID NO:3, the 5'end of the probe is marked with FAM, the 3'end of the probe is marked with a minor groove binder (MGB), and the positive control sample A comprises DNA plasmid of HBV genome fragments. The detection kit is suitable for quantitative detection of HBV nucleic acid in various tissues, cells, blood and other body liquid.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a fluorescent quantitative PCR kit for detecting hepatitis B virus nucleic acid, which is suitable for quantitative detection of hepatitis B virus nucleic acid in various tissues, cells, blood and other body fluids. Background technique [0002] Hepatitis B virus (HBV) infection not only causes acute and chronic viral hepatitis, but also is closely related to liver fibrosis and hepatocellular carcinoma. It is a public health problem that seriously endangers human health all over the world. About 2 billion people in the world have been proven to be infected with hepatitis B, and 350 million people are chronically infected. According to the report of the World Health Organization, among the top 10 causes of death in the world, hepatitis B ranks 9th. my country is a high-incidence area of ​​HBV infection in the world. About 130 million people in the country are HBV carriers, and there ...

AI Technical Summary

Problems solved by technology

nPCR is a qualitative detection method and cannot be directly quantified. Its products need to be qualitatively judged by gel electrophoresis; It can be judged by gel electrophoresis; the advantage of cPCR is that it can effectively control the difference in amplification efficiency between different reaction tubes, and the concentration of the target gene can be adapted to a large range, but this method requires gel electrophoresis, Southern blot and labeled isotope probes are used for quantitative analysis. Although the sensitivity is high, the detection conditions are not easy to standardize

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