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Porcine circovirus type 2 (PCV2) Cap protein gene and application thereof

A porcine circovirus and circovirus technology, applied in the fields of application, viral peptides, antiviral agents, etc., can solve problems such as the decline of immune protection of vaccines

Inactive Publication Date: 2012-12-19
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as porcine circovirus type 2 strains continue to mutate, the immune protection of existing vaccines continues to decline

Method used

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  • Porcine circovirus type 2 (PCV2) Cap protein gene and application thereof
  • Porcine circovirus type 2 (PCV2) Cap protein gene and application thereof
  • Porcine circovirus type 2 (PCV2) Cap protein gene and application thereof

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Embodiment Construction

[0011] The present invention will be further described below in conjunction with specific embodiments, but those skilled in the art should understand that the details and forms of the technical solution of the present invention can be modified or replaced without departing from the technical solution of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0012] 1. Amplification of porcine circovirus type 2 Cap protein gene

[0013] The clinically isolated and suspected PMWS disease materials were treated and inoculated with PCV1-free PK-15 cells. After three generations of blind passage, the full-length porcine circovirus type 2 gene was amplified by PCR. The primer sequences were as follows:

[0014] P1: 5′--GCTGGCTGAACTTTTGAAAG--3′

[0015] P2: 5′--AAATTTCTGACAAACGTTAC--3′

[0016] The amplification conditions were as follows: denaturation at 94°C for 5 minutes, 30 cycles at 94°C for 30S, 30S at 6...

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Abstract

The invention relates to a porcine circovirus type 2 (PCV2) Cap protein gene and an application thereof. The amino acid sequence of the protein is SEQ ID NO:1, and the nucleotide sequence of the protein is SEQ ID NO:2. The PCV2 Cap protein gene provided by the invention can be applied to preparation of a PCV2 Cap protein gene engineering subunit vaccine. The PCV2 Cap protein gene engineering subunit vaccine obtained in the invention is inoculated to five PCV2 negative weaned pigs of 21 days old through muscles, and another five weaned pigs are taken as a control group. Blood is sampled for detecting PCV2 antibodies 28 days after inoculation, which indicate that all PCV2 antibodies in an immune group are positive, the effective rate is up to 100 percent, and antibodies are not detected in the control group. As proved by a result, a prepared recombinant protein has high immunogenicity, and a further prepared PCV2 Cap protein gene engineering subunit vaccine can cause the immune response of piglets.

Description

technical field [0001] The invention belongs to the technical field of livestock genetic engineering vaccines, in particular to a porcine circovirus type 2 Cap protein gene and an application thereof. Background technique: [0002] Porcine Circovirus type 2 (PCV2) is the causative agent of Postweaning multsystemic wasting syndrome (PMWS), which can cause progressive weight loss, coughing, dyspnea, and diarrhea in weaned piglets , Pale or yellow skin, swollen lymph nodes, gray kidney edema and other symptoms. At the same time, the virus can also cause a certain degree of immunosuppression, which can easily cause secondary or concurrent infections of other diseases. Since clinical porcine circovirus was first discovered in Canada in 1991, the disease has caused huge economic losses to the swine industry all over the world. Therefore, the research on porcine circovirus type 2 has always been one of the key points in the field of poultry farming. [0003] The Cap protein of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/01C12N15/34A61K39/12A61P31/20
Inventor 王宏华于春梅凌红丽蒋皓静张园园
Owner QINGDAO VLAND BIOTECH INC