Packing agent for affinity chromatography
A filler and affinity technology, applied in chemical instruments and methods, solid adsorbent liquid separation, ion exchange, etc., to achieve high dynamic binding capacity and excellent pressure characteristics
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Examples
Embodiment 1
[0196] (i) Suspension polymerization of porous particles
[0197] Add 8.52 g of polyvinyl alcohol (PVA-217 manufactured by Kuraray Co., Ltd.), 2.13 g of sodium lauryl sulfate (EMAL10G manufactured by Kao Corporation), and 4.26 g of sodium carbonate to 4091 g of pure water, and stir overnight to prepare an aqueous solution (S-1- 1). Furthermore, another 30 g of (S-1-1) was extracted on the day of polymerization, and 2.13 g of sodium nitrite was dissolved in the remaining (S-1-1) to prepare an aqueous solution (S-2-1).
[0198] Next, 125 g of glycerin dimethacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.), 17.9 g of glycidyl methacrylate (manufactured by Mitsubishi Rayon Co., Ltd.), and 35.9 g of glycerin monomethacrylate (manufactured by NOF Corporation) were dissolved in 2 - 86.4 g of octanone (manufactured by Toyosei Co., Ltd.) and 253 g of acetophenone (manufactured by Wako Pure Chemical Industries, Ltd.) were prepared as a monomer solution. In addition...
Embodiment 2
[0240] In the suspension polymerization of Example 1, except that the stirring speed was set to 300 rpm, the same procedure was carried out to obtain porous particles 2, and the affinity chromatography formed by ligand-bonded porous particles having a ring-opening epoxy group was produced and evaluated. Filler 2 (A-2) was used. The results are shown in Table 2. The epoxy group content of the porous particles 2 was 0.45 mmol / g. (A-2) has a particle size of 31 μm, a pore volume of 1.438 mL / g, and a specific surface area of 132 m 2 / g, the volume average pore diameter is 110nm. The elastic modulus is 6.4MPa. The dynamic binding capacity of protein (human IgG antibody) is 40mg / mL, and the pressure flow rate is 1100cm / hr.
Embodiment 3
[0242] (i) Suspension polymerization of porous particles
[0243] Add 0.600 g of polyvinyl alcohol (PVA-217 manufactured by Kuraray), 0.030 g of sodium lauryl sulfate (EMAL10G manufactured by Kao Corporation), and 1.50 g of sodium sulfate to 300 g of pure water, and stir overnight to prepare an aqueous solution (S- 1-2). Furthermore, another 5 g of (S-1-2) was extracted on the day of polymerization, and 0.150 g of sodium nitrite was dissolved in the remaining (S-1-2) to prepare an aqueous solution (S-2-2).
[0244]Next, 8.19 g of glycerin dimethacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.), 3.51 g of glycidyl methacrylate (manufactured by Mitsubishi Rayon Co., Ltd.) were dissolved in 6.25 g of 2-octanone (manufactured by Toyosei Co., Ltd.) and phenylethyl ether. Ketone (manufactured by Wako Pure Chemical Industries, Ltd.) 18.3 g was prepared as a monomer solution. In addition, when the total mass of monomers is 100 parts by mass, the number of parts of...
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Abstract
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