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Kit and detection method for quickly screening mango-derived component in food and beverage

A technology of source components and detection methods, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as lack of determination methods

Active Publication Date: 2012-12-26
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no quick way to determine whether mango-derived ingredients are mixed in food

Method used

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  • Kit and detection method for quickly screening mango-derived component in food and beverage
  • Kit and detection method for quickly screening mango-derived component in food and beverage
  • Kit and detection method for quickly screening mango-derived component in food and beverage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Follow the procedure below for testing:

[0042] (1) Extraction of DNA from samples that may contain mango components

[0043] A. Weigh 0.1g sample and transfer it to a 1.5mL centrifuge tube. Add 600 μL of preheated lysis buffer, mix gently, and keep warm in a water bath at 65°C for 10 minutes;

[0044] B. Add an equal volume of 600 μL of phenol / chloroform into the tube, mix well by inverting up and down, and extract for 2 minutes;

[0045] C. Centrifuge at 12000g for 5min, draw the supernatant into a new centrifuge tube, add the same volume of sedimentation solution as the supernatant, mix well, leave at room temperature for 10min, centrifuge at 12000g for 5min, remove the supernatant, and keep the precipitate;

[0046] D. Add 60 μL of RNase to the precipitate, place it at 37°C for 2 minutes, mix it well with a pipette tip, dissolve the precipitate at 37°C, add 300 μL of buffer solution after 5 minutes, and mix up and down 10 times;

[0047] E. Take out the spin col...

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Abstract

The invention belongs to a kit and detection technology for quickly screening a mango-derived component in food and beverage. The invention is particularly characterized by using a real-time fluorescent PCR (polymerase chain reaction) technology to detect gene segments of a mango ITS2 gene. By using the real-time fluorescent PCR technology, the gene contained in a mango-derived component can be quickly, sensitively and specifically detected, so that whether the mango-derived component is contained in food and beverage can be screened. The quick screening kit and detection technology can be used for detecting the allergen mango component possibly contained in a compound sample, and can be also used for detecting whether a sample is free of the mango component. The method is quick, high in specificity, high in sensitivity, convenient to operate and good in reproducibility.

Description

technical field [0001] The invention belongs to the mango DNA detection technology of plant-derived components in food and beverages. Specifically, it uses real-time fluorescent PCR reaction technology to quickly screen and detect mangoes in food and beverages. The method includes DNA extraction, real-time fluorescent PCR amplification, etc. Increment and result determination. Its advantages are rapidity, strong specificity, high sensitivity, easy operation and good repeatability. Background technique [0002] Mango mango, scientific name Mangifera indica Linn., aliases: lemon fruit, mango fruit, boring fruit, honey hope, Wang fruit, face fruit and ampolo fruit, belong to Anacardiaceae (Anacardiaceae) mango genus. Mango is not only rich and sweet, but also helpful for beauty and weight loss. It is known as the "king of tropical fruits". There are more than 1,000 mango varieties in the world, and mangoes are often processed into mango juice, food, ice cream, yogurt, sugar, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 刘彩霞高宏伟孙敏龚方徐彪梁成珠
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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