Fusion protein capable of targeting activation of RANK signal pathway and recombinant plasmid and application thereof

Abstract

The invention discloses fusion protein capable of targeting activation of a RANK signal pathway, a recombinant plasmid and an application thereof. The fusion protein has an amino acid sequence as shown in SEQ ID NO. 1, and is obtained by substituting a segment of a gyrase B sequence for a C terminal of a mouse RANK; the nucleotide sequence coding the fusion protein is shown in SEQ ID NO. 2. After the fusion protein is introduced into a cell, continuous stimulation of a micromolecular medicament of coumermycin is accepted, and a RANK-dependent signal pathway is targetedly activated; and thus side effect caused by disease treatment though ligand-activated RANK signal pathway using acceptors is prevented. A recombinant vector contains the nucleotide sequence shown in SEQ ID NO. 2. The recombinant vector has very important value for RANK function research and RANK-caused disease treatment research.

Description

technical field [0001] The invention relates to the construction of a fusion protein, in particular to a fusion protein capable of targeting and activating the RANK signaling pathway, its recombinant plasmid and its application. Background technique [0002] The receptor activator of nuclear factor-κB (RANK) signaling pathway has multiple physiological functions. This signaling pathway is essential for tooth formation, lymph node formation, and osteoclast formation; the synergistic effect of RANK and CD40 signaling pathways can guide the formation of thymic medulla microstructure to control the body's central immune tolerance; RANK signaling pathway regulation Fever center; RANK signaling pathway can promote the survival and activity of dendritic cells; RANK signaling pathway is essential for the formation of mammary epithelial cells; RANK signaling pathway plays an important role in the generation of vascular endothelial cells and the formation of blood vessels; The RANK s...

AI Technical Summary

Problems solved by technology

However, activating the RANK signaling pathway with RANK ligands often causes many side effects, such as severe osteoporosis, fatal hypercalcemia, systemic inflammatory response, etc.

[0003] Therefore, it is necessary to transform RANK so that it not only retains the function of RANK, but also overcomes its defects of causing side effects

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