Haynaldia villosa 6VS chromosome specific molecular marker 6VS-UT and its application

Abstract

Relating to the technical field of molecular biology and genetic breeding science, the invention discloses a molecular marker 6VS-UT specifically tracing Haynaldia villosa 6VS chromosome and its application. In the invention, primers can be designed according to a wheat EST (expressed sequence tag) sequence BE445603 able to be electronically positioned on the short arm of chromosome 3 of brachypodium distachyon, corresponding DNA can be amplified and cloned from Haynaldia villosa and common wheat genomes, and based on sequencing, specific molecular marker primers can be further designed, with the concrete sequences shown as SEQIDNO.5 and SEQIDNO.6. When conducting PCR on the marker primers, a 301bp specific DNA band can be amplified, so that Haynaldia villosa 6VS chromosome and anti-wheat powdery mildew traits controlled thereby can be quickly and effectively traced. Thus, the Haynaldia villosa 6VS chromosome specific molecular marker 6VS-UT has a strong practical value in wheat anti-powdery mildew molecular marker assisted selective breeding.

Description

technical field [0001] The invention relates to the fields of molecular biology and genetic breeding science and technology, in particular to a molecular marker for specifically tracking the 6VS chromosome of T. villosa and its usage. Background technique [0002] Wheat is one of the three major food crops in the world, and it is also the first food crop in my country's total output. It is one of the important determinants of ensuring my country's food security. Wheat is often affected by biotic or abiotic stresses during its growth process. Wheat powdery mildew is a worldwide wheat disease. In recent years, with the improvement of farmland water conservancy facilities and water and fertilizer conditions in my country, its harm has been increasing year by year. The wheat-T6AL.6VS translocation line bred by distant hybridization and chromosome engineering technology shows high resistance or immunity to the currently discovered physiological races of powdery mildew, and its p...

AI Technical Summary

Problems solved by technology

However, in general, these markers have the disadvantage of instability in marker-assisted selection breeding, one reason is determined by the type of marker, such as RAPD marker OPT17 1400 and OPT17 1900 ; Secondly, due to the long marker sequence (mostly close to or exceeding 1000bp), the harsh requirements of the PCR reaction system and reaction conditions, and the poor reproducibility of PCR results

These shortcomings are not conducive to the application of marker-assisted selection technology in wheat breeding practice

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