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Diabetic retinopathy related gene polymorphism detection kit, and preparation method and purpose thereof

A technology of diabetic retina and gene polymorphism, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of no mature products and methods for diabetic retinopathy-related gene detection, etc.

Active Publication Date: 2013-01-02
EBIOS BEIJING BIOTECH
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] At present, there are no mature products and methods specifically for the detection of diabetic retinopathy-related genes

Method used

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  • Diabetic retinopathy related gene polymorphism detection kit, and preparation method and purpose thereof
  • Diabetic retinopathy related gene polymorphism detection kit, and preparation method and purpose thereof
  • Diabetic retinopathy related gene polymorphism detection kit, and preparation method and purpose thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] PCR pre-amplification of 23 SNP sites in peripheral blood samples of patients with diabetic retinopathy

[0048] 10ml of peripheral blood from a patient with diabetic retinopathy (with informed consent signed by the patient), and the genomic DNA in the peripheral blood of the patient was extracted according to the method of extraction and purification of genomic DNA from peripheral blood. The extracted genomic DNA is a multiplex PCR pre-amplification template, and the 1% (m / v) agarose gel dissolved by heating with 1×TAE buffer is used for electrophoresis to detect the DNA quality. figure 1 It shows that the genomic DNA extracted from 13 DR peripheral blood samples showed a single band after agarose gel electrophoresis without any bands, indicating that the PCR pre-amplification template was of high purity.

[0049] After the genomic DNA template of the DR peripheral blood sample was obtained, multiplex PCR pre-amplification was performed to enrich the extended template....

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Abstract

The invention provides a diabetic retinopathy related gene polymorphism rapid detection kit. The kit comprises multiplex PCR pre-amplification primers and single base extension primer sequences of 23 single nucleotide polymorphism (SNP) sites relates to diabetic retinopathy, a human peripheral blood genome DNA extraction liquid, multiplex PCR pre-amplification reaction liquid, a negative control, a positive control, a pre-amplification product purification reagent, PCR product single base extension reaction solution liquid, and a extension product purification reagent. The invention also provided a preparation method of the kit. According to the invention, diabetic retinopathy related SNP sites are detected by using a gene sequencing method with relatively high sensitivity and specificity, such that genotypes of the 23 diabetic retinopathy related gene polymorphism sites can be detected rapidly, effectively, accurately, and economically. Therefore, a reference is provided for diabetic retinopathy early diagnosis and clinical prognosis.

Description

technical field [0001] The invention relates to a kit for detecting related gene polymorphisms in early diagnosis of diabetic retinopathy and evaluation of clinical prognosis, as well as preparation of the kit and use in early diagnosis of diabetic retinopathy and evaluation of clinical prognosis. The present invention relates to 23 single nucleotide polymorphism (single nucleotide polymorphism, SNP) sites related to diabetic retinopathy, and 23 SNP site mutations or several mutations can diagnose diabetic retinopathy and evaluate the patient's clinical prognostic status. Background technique [0002] At present, diabetic retinopathy (DR) is one of the most common and serious microvascular complications of diabetes, and it is also a major blinding eye disease worldwide, which is the result of the joint action of genetic factors and environmental factors. At present, it can only be diagnosed by asking the clinical history and observing whether the patient has typical clinica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 曾沃坦焦剑金文俊梁辰陈初光
Owner EBIOS BEIJING BIOTECH
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