High-flux drug screening microfluidic chip

A microfluidic chip, high-throughput technology, applied in the direction of biomass post-treatment, biomass pre-treatment, biochemical cleaning device, etc., can solve the problems that cannot represent changes in physiological reactions, and improve detection accuracy and detection efficiency , the effect of reducing the cost of testing

Inactive Publication Date: 2013-01-16
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

The drug response in this way is carried out in a completely isolated state and cannot represent the physiological response changes caused by real drugs

Method used

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  • High-flux drug screening microfluidic chip
  • High-flux drug screening microfluidic chip
  • High-flux drug screening microfluidic chip

Examples

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Embodiment Construction

[0013] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0014] see Figure 1 to Figure 3 : A high-throughput drug screening microfluidic chip. Firstly, according to specific drug screening requirements, specific living cell fluorescent bioprobes are transfected into the target cells, incubated for a sufficient time, and the target cells transfected with the probes are added to the cell sampling component 3 of the chip, and then the target cells enter the buffer pool 4 , the target cells are sorted into single target cells through the disc-shaped curved channel 6, and then enter the plasmid transfection element 8, and the cell capture elements 9 of different specifications embedded in it capture different target cells and make them adhere to grow. Different drug concentrations are adjusted through the one-way driving microvalve 5 on the chip, and the drug to be screened is injected through the drug loading c...

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Abstract

The invention provides a high-flux drug screening microfluidic chip which is composed of a cell sample introduction element, a buffer pool, a one-way drive micro-valve, a disk-like bent channel, a drug loading element, a plasmid transfection element, a liquid receiving pool and the like. The elements for cell sample introduction, transfection, cell capture, drug loading and the like are assembled on one chip, so that the invention can simultaneously detect cell integral shape and intracellular labeled protein activity variation under different drug actions on one chip in the detection process, and monitor the action effect of the drug on the actual cells, thereby greatly lowering the detection cost, enhancing the detection accuracy and detection efficiency, and achieving the goal of drug screening.

Description

technical field [0001] The invention relates to a high-throughput drug screening chip, in particular to a high-throughput drug screening microfluidic chip. technical background [0002] The traditional drug screening chip mainly immobilizes the protein or nucleic acid molecule of the drug target on the chip through the principle of intermolecular interaction, and screens the drug by recording the reaction process of the combination of the drug molecule and the target molecule. The drug response in this way is carried out in a completely isolated state and cannot represent the physiological response changes caused by real drugs. Microfluidic chips have very powerful application value in many aspects such as drug screening, disease diagnosis, and environmental monitoring. Therefore, a new type of high-throughput drug screening microfluidic chip is currently needed. Contents of the invention [0003] The purpose of the present invention is to provide a simple structure, low...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
Inventor 廖晓玲刘波徐文峰
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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