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Zanthoxylum nitidum tissue culture medium

A tissue culture and culture medium technology, applied in the directions of plant cells, horticulture, and botany equipment and methods, can solve the problems of unfavorable large-scale production, high cost, difficult production operation, etc. good growth effect

Inactive Publication Date: 2013-07-24
钦州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If one culture medium is used for the provenance of L. chinensis from different origins or for a single plant of L. chinensis, the production operation will be difficult and costly, which is not conducive to large-scale production.

Method used

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  • Zanthoxylum nitidum tissue culture medium
  • Zanthoxylum nitidum tissue culture medium
  • Zanthoxylum nitidum tissue culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Liangmianzhen tissue culture medium, which includes subculture medium and rooting medium, wherein:

[0062] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0063] (1) Macroelements: potassium nitrate 1250mg / L, ammonium nitrate 1000mg / L, magnesium sulfate heptahydrate 370mg / L, calcium chloride dihydrate 166mg / L, potassium dihydrogen phosphate 150mg / L, disodium edetate 56.0mg / L, ferrous sulfate heptahydrate 41.7mg / L;

[0064] (2) Trace elements: manganese sulfate tetrahydrate 25mg / L, zinc sulfate heptahydrate 10mg / L, boric acid 10mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.083mg / L, copper sulfate pentahydrate 0.025mg / L L;

[0065] (3) Organic matter: inositol 100.0mg / L, glycine 2.0mg / L, thiamine hydrochloride (VB 1 ) 0.5mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, folic acid 0.5mg / L, biotin (VH) 0.05mg / L;

[0066] (4) Plant growth regulator: 6-benzyl adenine 0.4mg / L, naphthaleneacetic acid...

Embodiment 2

[0075] Liangmianzhen tissue culture medium, which includes subculture medium and rooting medium, wherein:

[0076] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0077] (1) Macroelements: potassium nitrate 1250mg / L, ammonium nitrate 1000mg / L, magnesium sulfate heptahydrate 370mg / L, calcium chloride dihydrate 166mg / L, potassium dihydrogen phosphate 150mg / L, disodium edetate 56.0mg / L, ferrous sulfate heptahydrate 41.7mg / L;

[0078] (2) Trace elements: manganese sulfate tetrahydrate 25mg / L, zinc sulfate heptahydrate 10mg / L, boric acid 10mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.083mg / L, copper sulfate pentahydrate 0.025mg / L L;

[0079] (3) Organic matter: inositol 100.0mg / L, glycine 2.0mg / L, thiamine hydrochloride (VB 1 ) 0.5mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, folic acid 0.5mg / L, biotin (VH) 0.05mg / L;

[0080] (4) Plant growth regulator: 6-benzyl adenine 1.0mg / L, naphthaleneacetic acid...

Embodiment 3

[0089] Liangmianzhen tissue culture medium, which includes subculture medium and rooting medium, wherein:

[0090] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0091] (1) Macroelements: potassium nitrate 1250mg / L, ammonium nitrate 1000mg / L, magnesium sulfate heptahydrate 370mg / L, calcium chloride dihydrate 166mg / L, potassium dihydrogen phosphate 150mg / L, disodium edetate 56.0mg / L, ferrous sulfate heptahydrate 41.7mg / L;

[0092] (2) Trace elements: manganese sulfate tetrahydrate 25mg / L, zinc sulfate heptahydrate 10mg / L, boric acid 10mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.083mg / L, copper sulfate pentahydrate 0.025mg / L L;

[0093] (3) Organic matter: inositol 100.0mg / L, glycine 2.0mg / L, thiamine hydrochloride (VB 1 ) 0.5mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, folic acid 0.5mg / L, biotin (VH) 0.05mg / L;

[0094] (4) Plant growth regulator: 6-benzyl adenine 0.6mg / L, naphthaleneacetic acid...

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Abstract

The invention discloses a zanthoxylum nitidum tissue culture medium. The medium comprises a subculture medium and a rooting medium. Improvement is carried out on the basis of an H medium, the macroelement usage is adjusted, and the iron vitriol content is increased. The medium is suitable for culturing zanthoxylum nitidum and single zanthoxylum nitidum tissues from different producing areas. The subculture period takes 20-25 days, the growth coefficient is 4-6, subculture seedlings grow well, small seedlings are thick and order, the leaf color is green, no etiolated seedlings are produced, and the seedling yield is high. The rooting rate of the rooted seedlings can reach 90%, the rooting is quick; and when the temperature is at 25 plus or minus 2 DEG C, the average rooting time is 10-15 days, a root system well grows, the rooting number is larger and averagely 3-5 roots, the seedling is good in lignification, the transplanting survival rate reaches above 90%, and the outplanting time of bottled seedlings is about 30 days averagely.

Description

technical field [0001] The invention relates to a tissue culture medium, in particular to a Liangmianzhen tissue culture medium. Background technique [0002] Zanthoxylum nitidum, a Rutaceae plant, is called "Zanthoxylum nitidum" because of the small barbs on both sides of the midrib of the leaves, also known as Liangbei needle, nail plate thorn (Fuzhou), entering mountain tiger, anesthetic vine, entering Ground Taurus, needles under the leaves, red barb tendons, big leaves and claws (Guangdong, Guangxi). Acupuncture likes to grow in warm and sunny places. It is more common in mountains, hills, flat forests, shrubs, and thorny shrubs on barren hills and grass slopes. It is mainly distributed in Guangdong, Guangxi, Fujian, Hainan, Yunnan, Guizhou and Taiwan and other places. Liangmianzhen is a kind of traditional Chinese medicinal material commonly used in my country. The rhizome, leaf and fruit peel can be used as medicine. Modern medicinal chemistry studies show that the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 时群陈丽文吴红英陈乃明何贵整邓月梅梁小娟谭冬晓蔡林梁刚谢云
Owner 钦州市林业科学研究所
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