The invention relates to a solanaceous fruit vegetable seedling substrate and a production method thereof. The solanaceous fruit vegetable seedling substrate is prepared from a main material, vermiculite and perlite, and is prepared by mixing the main material, the vermiculite and the perlite according to the volume ratio of (3 to 4) : (0.6 to 0.7) : (0.3 to 0.4) to obtain a mixture, adding 10 g to 15 g of paclobutrazol and 1.5 kg to 2 kg of dilute sulfuric acid solution of 0.4 mol / L to 0.5 mol / L to per cubic mixture, and mixing evenly; the main material is prepared from dairy cow bed removing material by adjusting a carbon/nitrogen ratio to be (25to 30): 1, a pH value to be 6.5-7.0, and water content to be 60% to 65%, inoculating EM fermentation bacteria, stacking, covering with a film, conducting high temperature aerobic fermentation, and air drying. By using the solanaceous fruit vegetable seedling substrate for seedling culture, a root system is developed and does not grow long, seedlings are robust, damping-off and root rot are significantly reduced, and aphid damage is reduced; the seedlings are planted in the field, virus diseases are relieved, and the yield is increased by 14% to 19%.