Tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides

Abstract

The invention discloses a tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides, which comprises the following steps: (1) inoculating an explant of the populus tremula multiplied by P. tremuloides on a start culture medium for culture after sterilization and obtaining an aseptic tube seedling; (2) cutting the tube seedling obtained in the step (1) into sections, and inserting into a differentiation culture medium for culture; (3) inserting the seedling sections obtained in the step (2) into a rooting culture medium for carrying out rooting culture; (4) transferring the seedling sections after rooting into the rooting culture medium for successive transfer culture; and (5) finally carrying out acclimatization and transplantation. The tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides has the advantages of rapid seedling growth rate, high survival rate, robust seedling, tender green leaf color, difficult vitrification, low production cost, industrialized production and the like.

Description

technical field [0001] The invention relates to a rapid propagation method of aspen hybrids, in particular to a tissue culture rapid propagation method of aspen hybrids, and belongs to the field of tissue culture of aspen hybrids. Background technique [0002] Populus tremula×P.tremuloides is a hybrid of Populus tremula×P.tremuloides, which is a hybrid of Populus tremula×P.tremuloides. It has the characteristics of fast growth, cold resistance, barren tolerance, tall tree shape, and strong adaptability. species. At the same time, the material of the hybrid is soft, dense, white, and the trunk is straight. It is a high-quality raw material for papermaking, plywood, particleboard and other industries. [0003] Studies have shown that: in Finland, European and American aspen hybrids are suitable for mountain afforestation, and plain afforestation grows better, and its rotation period and growth speed are faster than general mountain coniferous and broad-leaved afforestation sp...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the problems such as the high cost of the medium, the easy vitrification and browning of the tissue cultured seedlings, and the long emergence time of the commercial seedlings in the process of the hybrid tissue culture of Populus euramericana, and provide a new The European and American aspen hybrid tissue culture method has the advantages of low production cost, fast seedling growth, strong seedlings, not easy to vitrify, and high survival rate.

Images