Method for extracting and separating ginkgolide B from ginkgo leaves

A technology of ginkgolide and ginkgo leaf, applied in directions such as organic chemistry, can solve the problems of complex process of ginkgolide B, difficult to industrialized production, low yield and the like, and achieves good dispersibility, small solvent loss and high yield Effect
CN102887909AActive Publication Date: 2013-01-23黑龙江天宏药业股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
黑龙江天宏药业股份有限公司
Publication Date
2013-01-23
Patent Text Reader

Abstract

The invention discloses a method for extracting and separating ginkgolide B from ginkgo leaves. The method comprises the following steps of: extracting the ginkgo leaves with ethanol; performing reduced-pressure concentration of the extracting solution to obtain extract; adding water to dilute the extract, and adsorbing with a macroporous resin column; eluting with ethanol; performing reduced-pressure concentration on the eluent to obtain thick paste; uniformly mixing the thick paste with a diluent, and drying; packing the dry paste, eluting with ethyl acetate, and concentrating the ethyl acetate eluent until the smell of ethyl acetate does not exist; adding ethanol for dissolution; crystallizing with ethanol to obtain coarse crystal; and re-crystallizing the coarse crystal with ethanol and ethyl acetate to obtain the ginkgolide B with purity greater than or equal to 98%. According to the method provided by the invention, the technology is simple, the production cost is relatively low, the product purity is high, the yield is relatively high, and the method is favorable for industrial production.
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Description

technical field

[0001] The invention relates to a method for extracting ginkgolide B. Background technique

[0002] Ginkgolide is currently one of the most powerful and specific platelet activating factor (PAF) receptor blockers. Ginkgolide B has the strongest blocking effect on PAF receptor among ginkgolides, which can be eliminated in a dose-dependent manner. Platelet aggregation caused by PAF, thereby preventing the formation of thrombus. Pharmacological studies have shown that ginkgolide B has anti-allergic, anti-inflammatory, anti-shock effects, and also has protective effects on ischemic injury and organ transplant rejection. Ginkgolide B is relatively low in the commercially available Ginkgo biloba extract and has a specific structure. It is difficult to obtain high-purity and high-yield Ginkgolide B through extraction and separation.

[0003] The inventors have consulted domestic and foreign literature, and the authorized inventions or patents directly related to t...

Claims

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