Micro RNAs (ribonucleic acids) fluorescence detection probe

Abstract

The invention relates to a micro RNA (ribonucleic acid) fluorescence detection probe. The probe has a folding structure, and comprises a tail-end area and an annular area, wherein the tail-end area has a double-chain structure; the annular area has a single-chain structure and comprises a complementary sequence and an interval sequence of a target to be detected; and the interval sequence does not form a secondary structure with other sequences of the probe. By the tail-end area of the probe, the relative stability of the folding structure of the probe can be maintained; the folding structure is opened when a sample containing a detection object is added, a complementary primer of the tail-end area of the detection probe is added, and a deoxyribonucleic acid (DNA) polymerase without excision enzyme activity is added; micro RNA is released by extension of the primer and replacement of corresponding chains to allow the probe to recover and generate a fluorescence signal; the corresponding target micro RNA is detected by the generated detection fluorescence signal; and different fluorescence is marked according to the probes of the different RNAs, so that the simultaneous detection of the different micro RNAs can be realized.

Description

technical field [0001] The invention belongs to the fields of molecular biology and nucleic acid chemistry, and relates to a probe for simultaneously and sensitively detecting multiple microRNAs or short-segment RNAs. Background technique [0002] Studies have found that MicroRNAs (microRNAs) widely exist in eukaryotic cells. They are short-chain endogenous non-coding RNAs with important regulatory functions, usually 20-25 nucleotides in length. microRNAs are derived from longer RNA precursors containing folded structures. After synthesis, Dicer enzymes can recognize and cut them, and then form RNA-induced silencing complexes (miRISC, RNA-induced silencing complex) or other biological processes. The components of miRISC are microRNAs and protein Argonaute (Ago). RISC usually recognizes the 3' untranslated region (3'UTR) of the target gene mRNA by base pairing. When it perfectly matches the target gene mRNA, it will degrade the target gene mRNA; When paired, it inhibits the...

AI Technical Summary

Problems solved by technology

However, some of these methods require complex probe clusters, some require expensive materials, and the synthesis and modification of some nanoparticles will also bring higher cost and difficulty in operation.

In addition, because the homology of microRNA will lead to cross-interference in the detection of complex systems, this has great limitations for clinical practical application, especially for clinical diagnosis in the vast poverty-stricken areas of my country

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