Technique for planting mycorrhiza plant root bags in heavy metal contaminated soil

A technology of polluting soil and planting technology, applied in the field of environmental protection, can solve problems such as expensive engineering operation costs, slow growth of plants, and inability to survive, and achieve the effects of expanding the scope of nutrient absorption, promoting growth, and improving effects

Inactive Publication Date: 2013-02-13
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] High-concentration heavy metals pollute the soil. Because heavy metals are highly toxic to plants, plants often grow slowly or cannot survive. The technology of large-scale covering soil often requires a large amount of foreign aid to cover the soil and relatively expensive engineering operation costs

Method used

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  • Technique for planting mycorrhiza plant root bags in heavy metal contaminated soil
  • Technique for planting mycorrhiza plant root bags in heavy metal contaminated soil
  • Technique for planting mycorrhiza plant root bags in heavy metal contaminated soil

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Embodiment Construction

[0015] 1 Pure culture of ectomycorrhizal

[0016] The ectomycorrhizal fungus Laccaria amethystea (bu. Ex Gray) Murr (LA) is preserved by our laboratory. (1) Invert plate Prepare the MMN medium, mix well, and pour plate after autoclaving. The formula of MMN medium is: maltose 3g, glucose 10g, (NH 4 )HPO 4 0.25g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O0.15g,, CaCl 2 0.05g, FeCl 3 ·6H2O0.02g, NaCl0.025g, VB10.1mMg, agar 13g.

[0017] (2) Cutting a single bacterial block Select the MMN plate inoculated with LA, cut out a small piece of 5 mm × 5 mm at the edge of the colony, place it upside down on a new MMN plate, seal it, and incubate at 25°C for 1 month before use.

[0018] 2 Acquisition of mycorrhized plants

[0019] (1) Seedlings of Korean pine (Pinus koraiensis S.) are treated with 30% H 2 o 2 After sterilizing for 30 minutes, rinse with deionized water, then sow in vermiculite, and incubate at 25°C for 8 weeks before use.

[0020] (2) Mycorrhization Place a piece of filt...

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Abstract

The invention relates to the field of environmental protection and provides a novel planting method for revegetation in heavy metal contaminated places. The method is particularly suitable for revegetation in heavy metal contaminated soil which is obviously toxic for plant growth. Heavy metal in high-concentration heavy metal contaminated soil is quite toxic for plants, therefore the plants grow slowly or cannot survive, however, a large-scale earth covering technique requires for a large amount externally-aided covering soil and high project operation cost. According to the technique, a transplanting method of a root bag with soil is adopted, a small amount of non-contaminated soil is placed inside the root bag, a mycorrhiza plant is grown in the root bag, the root bag containing the soil and the plant is transplanted to the contaminated soil, therefore biomass and viability of the plant can be improved remarkably, the effects on absorbing and fixing soil heavy mental can be improved simultaneously, and the technique is particularly suitable for vegetation cover in high-concentration heavy metal contaminated soil which is obviously toxic for the plants.

Description

technical field [0001] The invention relates to the field of environmental protection, and is a novel plant planting method for vegetation recovery in heavy metal polluted land, and is especially suitable for phytoremediation of heavy metal polluted soil which is obviously poisonous to plant growth. Background technique [0002] At present, soil, as an important part of the ecological environment on which human beings depend, is increasingly polluted by heavy metals. The world's average annual emission of Hg is about 15,000 tons, Cu is 3.4 million tons, Pb is 5 million tons, Mn is 15 million tons, and Ni is 1 million tons. my country's soil heavy metal pollution is also quite serious, with millions of tons of toxic heavy metals released into the environment every year, including 346,000 tons of Pb and 39,000 tons of Cd (Nriagu and Pacyna, 1988). [0003] At present, the deterioration of the regional agricultural environment and the heavy metal pollution of agricultural prod...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
Inventor 陈亚华许璋阳沈振国夏妍王桂萍
Owner NANJING AGRICULTURAL UNIVERSITY
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