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Immunogen adherence and method of making and using same

A technology for mucosal and viral antigens, applied in the direction of egg immunoglobulin, antiviral agents, aerosol delivery, etc., can solve the problems of poor uniformity, mortality, average daily weight gain of pigs, etc.

Inactive Publication Date: 2013-02-13
CAMAS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This results in less uniform pigs, higher mortality, lower average daily gain, and fewer pigs born per litter

Method used

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  • Immunogen adherence and method of making and using same
  • Immunogen adherence and method of making and using same
  • Immunogen adherence and method of making and using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Selection of egg-laying females

[0044] The breed of laying hens can vary according to need and use. Any egg-laying female can be immunized, including chickens, turkeys, ducks, geese, pigeons, quails, ostriches, emu or any other bird. Regular breeds of laying hens are preferred and are usually selected based on the number of eggs laid per year, egg size and ease of housing. Rhode Island Reds, White Leghorns, and Red Sex Linked hybrids were animals selected based on egg size (large to extra large, 50-65 grams) and used in immunization programs. The ease of handling the animals and the size and consistency of the eggs, as well as the number of eggs laid per bird per year, were observed. Although any avian laying hen can be used, these chickens have proven to be the best performers due to cost and ease of use. White Leghorn, W98 mix had the best consistency and produced a greater number of eggs per animal. These animals produce large to extra-large eggs (50...

Embodiment 2

[0045] Example 2: Preparation of PM antigens for immunogens

[0046] Pasteurella multocida (ATCC 15743) was used as model bacteria. The organism was isolated from cattle. The ATCC method used to rehydrate the stock is as follows. Rehydrate bacteria in 1.0 mL of TSB. PM antigens on bacteria were stimulated using brain heart infusion (BHI, Acumedia). Stock TSB was inoculated into BHI broth and incubated at 37°C for 18-24 hours. This stimulates the formation of somatic and attached antigens on the bacteria. Flasks containing BHI broth were inoculated with BHI broth cultures. While stirring slowly, the flasks were incubated at 37°C. Blood agar plates were streaked to isolate colonies to confirm morphology. Good growth was seen after 22 hours. The flasks were combined and the resulting material was collected by centrifugation using sterile saline (0.9%) at approximately 3000 rpm for 30 minutes. The harvest was collected in test tubes. Density was checked using spectrophot...

Embodiment 3

[0047] Example 3: Preparation of PH antigens for immunogens

[0048] Pasteurella hemolytica stock (ATCC 14000) was used for the PH antigen. The organisms were isolated from cattle. Follow the ATCC method for rehydration stocks. Rehydrate bacteria in 1.0 mL of TSB. Brain heart infusion (BHI, Acumedia) was used to stimulate PM antigens on bacteria. Stock TSB was inoculated into BHI broth and incubated at 37°C for 18-24 hours. This stimulates the formation of somatic and attached antigens on the bacteria. Flasks containing BHI broth were inoculated with BHI broth cultures. While stirring slowly, the flasks were incubated at 37°C. Good growth was seen after 22 hours. Blood agar plates were streaked to isolate colonies to confirm morphology. The flasks were combined and the resulting material was collected by centrifugation using sterile saline (0.9%) at approximately 3000 rpm for 30 minutes. The harvest was collected in test tubes. Density was checked using spectrophotom...

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Abstract

A microbial adherence inhibitor in the form of fowl egg antibodies is disclosed, along with the method of making it and methods of using it. The inhibitor functions by substantially preventing the attachment of adherence of colony-forming immunogens in the respiratory tracts of host animals and humans. The inhibitor is made by inoculating female birds with the immunogen, harvesting the eggs which contain antibodies to the immunogen, and separating the yolk and albumin from the shells of the eggs. The yolk and albumin contents are administered to animals or human by distributing the contents directly or introducing the contents entrained in air. The adherence inhibiting material can be formulated for use in a variety of ways such as an oral spray or a nasal spray. These formulations can be effective to prevent or reduce respiratory illnesses in animals and humans.

Description

technical field [0001] The present invention relates to a microbial adhesion inhibitor in the form of avian egg antibodies for adequately preventing the adhesion or attachment of immunogens causing colony forming diseases in respiratory disease syndromes by inhibiting the ability of the immunogens to adhere to the mucous membranes of animals , the animals include host food animals, high-value non-food animals, zoological animals, companion animals, experimental animals or humans, and also relate to methods for preparing the adhesion inhibitor, and the use of the inhibitor. Background technique [0002] Microbiota form very complex interactions within the respiratory tract of animals. These animals may be dairy cows, breeding cattle, pigs and poultry such as chickens and turkeys, to name a few. Although the organisms may vary between different animal groups, they are basically bacteria (such as Pasteurellae, Mannhiemae, and Haemophilus), mycoplasma Genus (Mycoplasma) and re...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K9/12A61P11/00A61P31/00
CPCA61K39/395C07K16/02A61P11/00A61P31/00A61P31/12A61P31/16
Inventor 布拉德利·M·米特内斯康妮·菲利浦斯
Owner CAMAS INC
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