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Application of KLOTHO protein

A protein, application technology, applied in the field of biomedicine

Inactive Publication Date: 2013-03-13
黄曙 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching the domestic and foreign literature and patents of the prior art, there is no research report on the use of KLOTHO protein and its related components in the diagnosis and treatment of hepatocellular carcinoma

Method used

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  • Application of KLOTHO protein
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  • Application of KLOTHO protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 The clinical significance of KLOTHO protein expression in cancer tissue of patients with liver cancer is lower than that in paracancerous tissue

[0025] Immunohistochemical S-P method was used to detect the expression of KLOTHO protein in tissue microarrays composed of 52 cases of liver cancer and 54 cases of paracancerous tissues, and combined with clinicopathological data for statistical analysis. The result is as figure 1 , figure 2 , Tables 1 and 2 show that the positive rate of KLOTHO protein expression in liver cancer tissues is lower than that in paracancerous tissues ( P P P >0.05).

[0026] The survival analysis of the liver cancer patients included in the study found that the positive expression of KLOTHO protein was positively correlated with the prognosis of the patients. The KLOTHO protein-negative group had a longer survival time (mean survival time was 16.772 months, 95% confidence interval CI was 11.550-21.993), and the Log-rank test showe...

Embodiment 2

[0032] Example 2 Confirming the role of klotho in the occurrence and progression of liver cancer at the level of cell models

[0033] (1) Transfection of klotho high expression vector inhibits the growth of liver cancer cells and promotes the apoptosis of liver cancer cells

[0034] like image 3 As shown, after 48 hours of transfection of the high klotho expression vector pCMV6-Entry-KL (its control empty vector pCMV6-Entry) in the liver cancer cell HepG2, the expression level of KLOTHO protein was detected by Western Blot to verify the effect of the high expression vector. The results showed that the liver cancer cell KLOTHO protein expression was significantly up-regulated in HepG2, * P < 0.05, indicating that the klotho high expression vector can effectively up-regulate the expression of KLOTHO protein.

[0035] HepG2 cells were transfected with klotho high-expression vector pCMV6-Entry-KL (its control empty vector pCMV6-Entry) 48 hours later, cell proliferation was de...

Embodiment 3

[0041] Example 3 Confirming the inhibitory effect of KLOTHO protein on the growth of liver cancer at the animal model level

[0042] Ten BALB / cASlac-nu nude mice aged 6-8 weeks, half male and half male (purchased from the Animal Experiment Center of Nanjing Medical University, Jiangsu Province). The amount of cells injected subcutaneously in the back of each nude mouse was 2×10 6 200μl / 100μl HepG2 cells were observed for 2 weeks. Eight nude mice with successful tumor formation were randomly divided into two groups, one group was injected with normal saline (Control group) intraperitoneally, and the other group was injected with exogenous secreted KLOTHO protein solution intraperitoneally. Injections were given every 2 days for a total of 3 injections. After the last injection, observe continuously for 5 weeks, and measure the size of the tumor-bearing nude mouse liver cancer tissue every week (as shown in Table 3); Figure 10 and Table 3), it was found that after treatment ...

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Abstract

The invention provides application of a KLOTHO protein in preparation of medicines for treating liver cancer. An amino acid sequence of the KLOTHO protein is shown in SEQ NO.1; and the invention also provides application of the KLOTHO protein in preparation of formula for diagnosing the liver cancer, and the amino acid sequence of the KLOTHO protein is shown in SEQ NO.1. The application of the KLOTHO protein has the following technical effects that: (1) the prediction occurrence of the liver cancer is reduced by detecting expressions of individual liver tissues and (or) KLOTHO proteins in blood and related composition components because the KLOTHO protein expression in the cancer tissues of a liver cancer patient is lower than para-carcinoma tissues; (2) the liver cancer is treated by improving the expression level of the KLOTHO proteins and related composition components in the liver cancer tissues because the growth of the liver cancer is inhibited by the KLOTHO proteins; and (3) because the KLOTHO participates a pathological process related to cell activities of malignant proliferation, apoptosis, invasion and the like, experience and application base is provided to deeply discuss about the relation between KLOTHO and other diseases for the future.

Description

technical field [0001] The invention provides the use of KLOTHO protein, which belongs to the technical field of biomedicine. Background technique [0002] Primary hepatocellular carcinoma (referred to as liver cancer, HCC) is one of the most common malignant tumors worldwide. According to data released by the American Cancer Society (ACS) in December 2007, there were 667,000 cases of liver cancer and 598,000 deaths per year. Among them, about 50% of new cases and deaths are in China, that is, liver cancer poses a greater threat to the health of our people and has become the second cancer killer in our country. , society brings serious economic and psychological burden. [0003] Klotho is an aging-related gene discovered in 1997. It is located on human chromosome 13q12 and is about 50kb in length, consisting of 5 exons and 4 introns. The cDNA of the Koltho gene contains two different fragments, which encode two different proteins: one is the membrane type, which is locate...

Claims

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Application Information

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IPC IPC(8): A61K38/22A61K38/47G01N33/68A61P35/00
Inventor 黄曙汤小伟季国忠范志宁王敏
Owner 黄曙
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