Unlock instant, AI-driven research and patent intelligence for your innovation.

Trypsin-regulated nanometer supramolecular vesicles and preparation method and application thereof

A trypsin and supramolecular technology, applied in the field of preparation of nano-supramolecular materials, can solve complex problems, reduce biocompatibility and enzyme reactivity, increase preparation costs, etc., and achieve simple preparation methods, good selective responsiveness, and good stability sexual effect

Inactive Publication Date: 2014-04-09
NANKAI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, introducing enzyme-responsive sites into vesicle systems usually requires complicated and time-consuming covalent linkage synthesis, which not only increases the cost of preparation, but also introduces organic solvents and toxic reagents into the vesicle system during the synthesis process. which reduces its biocompatibility and enzymatic activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trypsin-regulated nanometer supramolecular vesicles and preparation method and application thereof
  • Trypsin-regulated nanometer supramolecular vesicles and preparation method and application thereof
  • Trypsin-regulated nanometer supramolecular vesicles and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] A method for preparing nano supramolecular vesicles regulated by trypsin, dissolving sulfonated calix[4]arene and protamine in water and uniformly mixing them to obtain the supramolecular vesicle solution target, the sulfonated calix [4] The concentrations of aromatic hydrocarbons and protamine are 0.02mmol / L and 50μg / mL respectively, and then adding trypsin can completely depolymerize the supramolecular vesicles in the supramolecular vesicle solution. The concentration in the vesicle solution was 0.2 mg / mL. figure 1 Schematic diagram for the construction of supramolecular assemblies through host-guest inclusion coordination interactions.

[0030]Sulfonated calix[4]arenes cannot self-aggregate in aqueous solution in the absence of protamine, see: M. Rehm, M. Frank, J. Schatz. Tetrahedron Lett. 2009, 50, 93-96; eg figure 2 As shown, the presence of protamine can induce the amphiphilic aggregation of sulfonated calix[4]arene in aqueous solution. When the concentration o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses trypsin-regulated nanometer supramolecular vesicles. According to a building unit, a sulfonated cup [4] aromatic hydrocarbon serves as a host, protamine serves as a guest, and a supramolecular assembling body is built through host-guest inclusion complexation interaction. The preparation method comprises the following steps of: dissolving the sulfonated cup [4] aromatic hydrocarbon and the protamine in water, and uniformly mixing to obtain a target of a supramolecular vesicle solution; and adding trypsin into the target, so that supramolecular vesicles in the supramolecular vesicle solution are completely depolymerized. The method has the advantages that the preparation method of the nanometer supramolecular vesicles is easy and convenient, and raw materials of the host and the guest are less; the nanometer supramolecular vesicles have high biocompatibility and stability and have high response selectivity on the trypsin; and moreover, the supramolecular vesicles can be loaded with hydrophilic broad spectrum anti-cancer medicinal molecule adriamycin, and the loaded adriamycin can be released to a targeting locus of the trypsin with high selectivity and has wide application prospect in the fields of targeting drug delivery and treatment of pancreatic cancer.

Description

【Technical field】 [0001] The invention belongs to the preparation of nano-supramolecular materials, in particular to a nano-supramolecular vesicle regulated by trypsin, a preparation method and application thereof. 【Background technique】 [0002] Stimuli-responsive vesicles have received extensive attention in recent years because of their important application values ​​in the fields of chemistry, biology, and material science. See: 1) G. Fuks, R.M.Talom, F.Gauffre.Chem.Soc.Rev. 2011, 40, 2475-2493; 2) D.M. Vriezema, M.C. Aragonès, J.A.A.W. Elemans, J.J.L.M. Cornelissen, A.E. Rowan, R.J.M. Nolte. Chem. Rev. 2005, 105, 1445–1489; 3) X. Guo, F.C. Szoka, Jr.A . Chem. Res. 2003, 36, 335–341. The use of stimuli-responsive vesicles to load drugs and then release the loaded drugs at specific signal-responsive sites has become a research hotspot, because targeted drug delivery can improve the efficacy of loaded drugs and at the same time Reduce the toxic and side effects of drugs....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K9/50A61K31/704A61P35/00A61K47/69
Inventor 刘育王魁郭东升赵梦尧
Owner NANKAI UNIV