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Method for extracting DEHP (di(2-ethylhexyl)phthalate) from fermentation product of ocean paenibacillus polymyxa L1-9 and application

A technology for polymyxa spores and fermentation products, which is applied in the extraction of DEHP from the fermentation products of marine Paenibacillus polymyxa L1-9, the application field of the aforementioned DEHP, and can solve problems such as no research reports on Paenibacillus polymyxa.

Inactive Publication Date: 2014-09-10
HUAIHAI INST OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] The Paenibacillus polymyxa that has been studied is mainly isolated from the soil, and a few are plant endophytes, all of which are in terrestrial environments. There are no research reports on Paenibacillus polymyxa from the ocean

Method used

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  • Method for extracting DEHP (di(2-ethylhexyl)phthalate) from fermentation product of ocean paenibacillus polymyxa L1-9 and application
  • Method for extracting DEHP (di(2-ethylhexyl)phthalate) from fermentation product of ocean paenibacillus polymyxa L1-9 and application
  • Method for extracting DEHP (di(2-ethylhexyl)phthalate) from fermentation product of ocean paenibacillus polymyxa L1-9 and application

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Embodiment 1

[0072] Embodiment 1, a method for extracting DEHP from a marine Paenibacillus polymyxa L1-9 fermentation product, the steps are as follows:

[0073] (1) L 1The preparation of -9 bacterial strain fermented liquid: use fermentor to L 1 -9 strains were fermented in batches, and the fermentation conditions were: the bacterial age of the seed liquid was 24h, and the concentration was 10 9 cells / mL, inoculum size 8%, temperature 28°C, pH 6.3, initial stirring speed 250r / min, ventilation rate 3L / min, fermentation time 36h; 1 -9 strain fermentation broth; L 1 After centrifugation at 10,000r / min, the fermentation broth of -9 strains was tested for antibacterial activity against Fusarium graminearum with 50 μl / well, and the L 1 -9 strain fermentation broth was initially extracted;

[0074] (2) Preliminary extraction of antibacterial active substances: L 1 The fermented liquid of strain -9 was extracted twice with an equal volume of ethyl acetate, and after standing for 12 hours, th...

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Abstract

The invention discloses a method for extracting DEHP (di(2-ethylhexyl)phthalate) from a fermentation product of ocean paenibacillus polymyxa L1-9. The method takes fusarium graminearum as an indicator and performs separation and purification of the antibacterial-activity components generated by the strain of paenibacillus polymyxa L1-9 by means of column chromatography on silica gel, SephadexLH-20 column chromatography, HPLC (high performance liquid chromatography) and the like to obtain an antifungal compound B2; and according to the structural identification by the spectrum technologies such as ESI-MS, <1>HNMR, <13>CNMR and the like, B2 is DEHP. The method disclosed by the invention separates DEHP from the metabolic product of paenibacillus polymyxa; and experiments prove that DEHP has an obvious inhibition effect on fusarium graminearum. The bacteria have the advantages that the growth period is short, large-scale culture is easy to implement in a laboratory, the fermentation product is easier to purity and the like, and are favorable for large-scale fermentation to realize industrial production.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from marine bacteria fermentation products, especially a marine Paenibacillus polymyxa L 1 -9 A method for extracting DEHP from a fermentation product, the present invention also relates to the use of the aforementioned DEHP. Background technique [0002] Paenibacillus polymyxa ( Paenibacillus polymyxa ) as important plant disease biocontrol bacteria and plant rhizosphere growth-promoting bacteria have been widely used in the agricultural field. Paenibacillus polymyxa can produce a variety of antibacterial substances such as peptides, proteins, nucleosides, pyrazines and phenols. For example, the protein produced by Paenibacillus polymyxa VLB16 can inhibit the rice blast fungus ( Magnaporthe oryzae ) and Rhizoctonia solani ( Rhizoctonia solani ) growth, its molecular weight is 37KDa, and it can withstand the high temperature of 121 ℃. Beatty et al. isolated a cluster of antibacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C07C69/80C07C67/56A01N37/10A01P3/00C12R1/01
Inventor 暴增海马桂珍吴少杰王淑芳付泓润葛平华
Owner HUAIHAI INST OF TECH
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