Hydnocarpus hainanensis anti-tumor effective component extractive as well as preparation method and application thereof
A technology of effective components and anti-tumor drugs, which is applied in the field of anti-tumor active components of Hainan Dafengzi extract, and can solve the problems of not involving the antitumor active parts of Dafengzi.
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Embodiment 1
[0022] Example 1. Preparation of the extract of the anti-tumor effective components of Fructus hainanensis
[0023] Dehull 5kg of the fruit of Hainan Dafengzi, remove the oil from the pulp with a press machine, crush the fruit shell and combine it with the oil-removed medicinal residue. Reflux extraction with 95% ethanol for 2 hours, the amount of ethanol is 3 times the mass of Dafengzi, the filtered residue is extracted once again, filtered, combined with 30L of ethanol extracts twice, recovered ethanol until almost no alcohol smell, continue to concentrate to 600ml, Add water to disperse, add an equal volume of ethyl acetate to extract 4 times, combine 2400ml of ethyl acetate extract, recover ethyl acetate, and obtain about 100g of ethyl acetate extract. Take 20 g of ethyl acetate extract, pass through a silica gel column, and use dichloromethane and dichloromethane-methanol (100:1, 50:1, 20:1) as eluents to elute in sequence. Each eluate was concentrated under reduced pres...
Embodiment 2
[0024] Example 2. Preparation of the extract of the anti-tumor effective components of Fructus hainanensis
[0025] Crush 5kg of the fruit of the fruit of the hainan chinensis, soak it in petroleum ether (60-90 ℃) for 2 hours, and the amount of petroleum ether should be twice the mass of the hainan fruit. Remove the lower layer of medicinal dregs, and soak again with petroleum ether for 2 hours. The lower layer of medicinal residue was reflux extracted with 80% ethanol for 3 hours. The amount of ethanol was 3 times the mass of Dafengzi. The filtered residue was extracted once again, filtered, and the ethanol extracts were combined twice. The ethanol was recovered until almost no alcohol smell, and continued to concentrate to 600ml, add water to disperse, add an equal volume of ethyl acetate to extract 4 times, combine 2400ml of ethyl acetate extract, recover ethyl acetate, and obtain about 100g of ethyl acetate extract. Take 20 g of ethyl acetate extract, pass through a silic...
Embodiment 3
[0027] Example 3. Preparation of the extract of the anti-tumor effective components of Fructus hainanensis
[0028] Grind 5 kg of fruit of Fructus hainanensis, extract with 95% ethanol under reflux for 2 hours, the amount of ethanol is 3 times of the mass of Fructus Rhizoma hainanensis, extract the residue after filtration once, filter, combine the ethanol extracts twice, and recover the ethanol until almost no alcohol smell, continue to concentrate to 600ml, add water to disperse, and add an equal volume of petroleum ether to extract 3 times. The aqueous layer was extracted 6 times with an equal volume of ethyl acetate, the ethyl acetate extracts were combined, and the ethyl acetate was recovered to obtain about 100 g of the ethyl acetate extract. Take 20 g of ethyl acetate extract, pass through a silica gel column, and use dichloromethane and dichloromethane-methanol (100:1, 50:1, 20:1) as eluents to elute in sequence. Each eluate was concentrated under reduced pressure and...
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