Method for preparing anti-cropping ecological fungicide for repairing panax notoginseng continuous cropping soil

A technology for resisting continuous cropping and Panax notoginseng is applied in the field of microbial fermentation to achieve the effects of shortening fermentation cycle, improving equipment utilization efficiency and reducing costs

Inactive Publication Date: 2013-04-17
NANKAI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The use of bacteria-fungi combined to prevent the root-growing fungus of Panax notoginseng and its high-density, high-activity production process have not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the probiotic bacterial agent, including the fermentation step, is to inoculate the production secondary and tertiary bacterial liquid of the bacterial agent in the culture medium with corn flour-potato-soybean meal as the main component to carry out mixed and expanded culture and fermentation. The fermentation process is an aerobic mixed fermentation at a temperature of 30°C and a pH value of 7.2-7.6. The production strains are Bacillus subtilis, Bacillus licheniformis, Bacillus polymyxa, Streptococcus faecalis and Candida tropicalis , Paecilomyces lilacinus, Streptomyces luteus and other 7 strains of bacteria-fungi. A solid bacterial agent that adsorbs and immobilizes the end product of fermentation on peat biochar.

[0023] Concrete process steps and process parameters involved in the present embodiment are:

[0024] (1) After activating the preserved strains on the slant by streaking on a plate, pick a single colony, and expand and cultiva...

Embodiment 2

[0031] Inoculate the bacteria of the production strain of the beneficial root bacterium into the beef extract peptone medium, and the fungus inoculates

[0032] In the potato-sucrose medium, the first grade was prepared at a temperature of 30°C and a pH value of 7.6.

[0033] Seed culture fluid. The secondary and tertiary expanded cultivation uses corn flour-potato-soybean meal as the main ingredients

[0034] Divided culture medium. Production bacterial classification is selected with embodiment 1.

[0035] Concrete process steps and process parameters involved in the present embodiment are:

[0036] (1) After activating the preserved slant strains with the method of streaking on the plate, pick a single colony and expand the culture system step by step through test tubes (7mL), small triangular flasks (100mL) and large triangular flasks (1500mL) respectively. into a first-class seed solution (each strain of 1500mL); the preparation process of the first-class seed solutio...

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Abstract

The invention discloses a method for preparing a probiotic fungicide for preventing panax notoginseng continuous cropping diseases, belonging to the field of microbial fermentation. The method comprises the steps of fermenting, namely respectively culturing individual production strains of the probiotic fungicide into a primary seed solution, inoculating to a culture medium which takes corn flour-potato-bean pulp as a principle component for performing aerobic mixed fermentation and enlarged cultivation, wherein the production strains are selected from seven bacteria-fungi such as bacillus subtilis; and adsorbing and immobilizing the fermentation end products to turf charcoal so as to prepare a solid fungicide. The continuous cropping diseases which are ubiquitous in the conventional panax notoginseng plantation technology are solved, and continuous cropping and continuous harvest can be realized in the panax notoginseng planting fields after the panax notoginseng is planted for 2-3 years. The preparation method has the outstanding characteristics of low production cost, short fermentation period and high flora density. According to the fungicide, dead seedlings, yellow seedlings, wither, dried leaves, root rot and other ecological soil symptoms in the panax notoginseng planting process can be prevented; and meanwhile, according to the method, root growth can be promoted, the soil is loosened, strong seedlings are cultivated, and pesticide and fertilizer residues are removed and the obvious function of ecologically repairing the soil is achieved.

Description

Technical field [0001] The invention belongs to microbial fermentation, in particular to a preparation method of an ecological bacterial agent capable of resisting heavy cropping and restoring the soil of Panax notoginseng continuous cropping. Background technique [0002] Today, when Panax notoginseng is planted in large quantities, due to the extensive use of chemical fertilizers and pesticides, the soil ecosystem formed by soil-microorganisms-Sanqi roots has been severely damaged, beneficial bacteria have been inhibited, and pathogenic fungi, such as rust rot bacteria, bad blight Solanum, Fusarium solani, Rhizoctonia solani and Pythium etc. spread and grow, causing frequent cropping diseases of Panax notoginseng, such as root rot, withering, yellow seedlings, mosaic leaves, black spots, etc. The forest soil cannot be reused after planting 3-5 years (one crop) of Panax notoginseng. Generally, it takes ten years or even more than ten years of natural restoration before Pana...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C09K17/14C12R1/125C12R1/10C12R1/12C12R1/46C12R1/74C12R1/79C12R1/465C09K101/00
Inventor 张清敏唐景春张彦峰王发云王麟猛赵颖张莹捷
Owner NANKAI UNIV
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