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Low-temperature complex bacterial preparation for microbial storage of dry and yellow corn stalks and application of preparation to production of dry and yellow corn stalk feed

A compound bacterial agent and dried corn technology is applied in the field of microorganisms to achieve the effects of improving safety, reducing the number and increasing the number of lactic acid bacteria

Active Publication Date: 2014-05-07
黑龙江久佳农业科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that there is no microbial agent used in the low temperature season in the north that can quickly promote the low-temperature micro-storage process of dry corn stalk feed, and to provide a low-temperature fermentation bacterial agent for preparing dry corn stalk feed

Method used

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Experimental program
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Effect test

specific Embodiment approach 1

[0015] Specific embodiment one: The low-temperature composite microbial agent for the micro-storage of dried corn yellow stalks in this embodiment includes 15 parts of Lactobacillus flexus ( Lactobacillus curvatus ) DSM 20010 fermentation broth, 25 parts of Lactobacillus Nordensis ( Lactobacillus nodensis ) JCM 14932 fermentation broth, 25 parts Lactobacillus sake ( Lactobacillus sakei ) ATCC 15521 fermentation broth and 10 parts of Lactobacillus kimchi ( Lactobacillus kimchii ) DSM 19674 fermentation broth; the number of effective viable bacteria in the fermentation broth of the above four bacteria is 10 9 Individuals / mL or more.

[0016] The steps of the preparation method of the low-temperature composite bacterial agent are as follows:

[0017] 1. Preparation of bacterial agents: Inoculate the preserved Lactobacillus flexus DSM 20010, Lactobacillus Nordensis JCM 14932, Lactobacillus sake ATCC 15521, and Lactobacillus kimchi DSM 19674 into MRS-S medium respectively, an...

specific Embodiment approach 2

[0021] Specific embodiment two: The low-temperature composite microbial agent for the micro-storage of corn dry yellow stalks in this embodiment includes 30 parts of Lactobacillus flexus DSM 20010 fermentation broth and 50 parts of Lactobacillus Nordensis JCM 14932 fermentation broth in parts by weight , 30 parts of Lactobacillus sake ATCC 15521 fermentation broth and 30 parts of Kimchi Lactobacillus DSM 19674 fermentation broth; the effective number of viable bacteria in the fermentation broth of the above four bacteria is 10 9 Individuals / mL or more. The preparation method of the low-temperature composite microbial agent of this embodiment is the same as that of Embodiment 1.

[0022] The low-complex bacterial agent can be used to prepare dried corn stalk feed, and can quickly colonize, produce lactic acid, and reduce the pH of the fermentation system in a corn stalk fermentation system without adding any soluble sugar and at an ambient temperature of about 10°C. Value and ...

specific Embodiment approach 3

[0024] Specific embodiment three: The low-temperature compound microbial agent for the micro-storage of dried corn yellow stalks in this embodiment includes 28 parts of Lactobacillus flexus DSM 20010 fermentation broth and 45 parts of Lactobacillus Nordensis JCM 14932 fermentation broth in parts by weight , 29 parts of Lactobacillus sake ATCC 15521 fermentation broth and 27 parts of Kimchi Lactobacillus DSM 19674 fermentation broth; the effective number of viable bacteria in the fermentation broth of the above four bacteria is 10 9 Individuals / mL or more. The preparation method of the low-temperature composite microbial agent of this embodiment is the same as that of Embodiment 1.

[0025] The low-complex bacterial agent can be used to prepare dried corn stalk feed, and can quickly colonize, produce lactic acid, and reduce the pH of the fermentation system in a corn stalk fermentation system without adding any soluble sugar and at an ambient temperature of about 10°C. Value and...

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Abstract

The invention belongs to the field of microbiology, and relates to a low-temperature complex bacterial preparation for microbial storage of dry and yellow corn stalks, and application of the preparation to production of a dry and yellow corn stalk feed, which overcome the lack of bacterial preparations for quickly promoting a low-temperature microbial storage process of a dry and yellow corn stalk feed in low-temperature seasons in north China at present. The low-temperature complex bacterial preparation comprises the following components in parts by weight: 15-30 parts of lactobacillus curvatus DSM20010 fermentation broth, 25-50 parts of lactobacillus nodensis JCM14932 fermentation broth, 25-30 parts of lactobacillus sake ATCC15521 fermentation broth and 10-30 parts of lactobacillus pickle DSM19674 fermentation broth. The low-temperature complex bacterial preparation can promote the low-temperature microbial storage process of the dry and yellow corn stalks, and improve the quality and the palatability of the fermented feed.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a low-temperature composite bacterial agent made from a variety of microbial fermentation liquids. Background technique [0002] Although my country is a large agricultural country, it has a large population and little land, insufficient grain production, and a shortage of feed grain. However, with the continuous development of agriculture and animal husbandry and the improvement of people's living standards, the grain used to produce feed has risen to 2 billion tons, the contradiction between humans and animals competing for food is seriously affecting the economic balance of our country. In addition, although the grassland area is large, due to natural disasters and man-made disasters, the grassland is seriously degraded, and the shortage of forage has become one of the problems restricting the development of animal husbandry. my country's straw resources are extremely...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 王彦杰王伟东王翠晏磊高亚梅
Owner 黑龙江久佳农业科技有限公司
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